Adenine nucleotide translocase 3 (ANT3) overexpression induces apoptosis in cultured cells

被引：86

作者：

Zamora, M ^{[1
]}

Granell, M ^{[1
]}

Mampel, T ^{[1
]}

Viñas, O ^{[1
]}

机构：

[1] Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

来源：

FEBS LETTERS | 2004年 / 563卷 / 1-3期

关键词：

ADP/ATP carrier; adenine nucleotide translocase; mitochondrion; apoptosis; cell death;

D O I：

10.1016/S0014-5793(04)00293-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis [Bauer et al. (1999) J. Cell Biol. 439, 258-262]. Nothing is known, however, about the apoptotic activity of ANT3. We have transfected HeLa cells with the three human ANT isoforms to compare their potential as inducers of apoptosis. Transient overexpression of ANT3 resulted, like ANTI, in apoptosis as shown by an increase in the sub-G1 fraction, annexin V staining, low DeltaPsi(m), and activation of caspases 9 and 3. Moreover, the apoptosis produced by ANT3 was inhibited by bongkrekic acid and by cyclosporin A. The pro-apoptotic activities of the ANT1 and ANT3 isoforms contrast with the lack of apoptotic activity of ANT2. This finding may help to identify the specific factors associated with the pro-apoptotic activities of ANT isoforms. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：155 / 160

页数：6

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