In vitro inhibition of Escherichia coli O157:H7 by bifidobacterial strains of human origin

被引:77
作者
Gagnon, M
Kheadr, EE
Le Blay, G
Fliss, I
机构
[1] Univ Laval, Dept Sci Aliments & Nutr, Ctr Dairy Res, STELA & Nutraceut & Funct Foods Inst, Quebec City, PQ G1K 7P4, Canada
[2] Univ Alexandria, Fac Agr, Dept Dairy Sci & Technol, Alexandria, Egypt
基金
加拿大自然科学与工程研究理事会;
关键词
probiotics; bifidobacteria; Escherichia coli O157 : H7; adhesion; caco-2; cells; antagonism;
D O I
10.1016/j.ijfoodmicro.2003.07.010
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The ability of bifidobacteria isolated from infant feces to inhibit enterohemorrhagic Escherichia coli serotype O157:117 in vitro and reduce its adhesion to human enterocyte-like Caco-2 cells was evaluated in comparison to American Type Culture Collection bifidobacterial reference strains. Five Bifidobacterium isolates from infant feces were identified and characterized by morphology, fructose-6-phosphate phosphoketolase (F6PPK) assay, polymerase chain reaction using bifidobacterial 16S rDNA specific primers, carbohydrate fermentation patterns, resistance to lysozyme, acid, bile and hydrogen peroxide as well as their ability to inhibit E. coli O157:147 using the agar spot technique. Infant isolates showed greater resistance to bile, acid, lysozyme and more antimicrobial activity against E. coli O157:H7 than ATCC strains. Two infant isolates identified as B. bifidum RBL 71 and B. bifidum RBL 460 showed good adhesion and significant potential for reducing adhesion of E. coli O157:H7 to Caco-2 cells. This effect was dependent on bifidobacterial cell concentration. These results show that bifidobacteria isolated from infants may be useful for improving probiotic formulae with respect to protection against E. coli O157:117 infection. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:69 / 78
页数:10
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