Multicentre European study comparing selection techniques for the isolation of CD34+ cells

被引:27
作者
de Wynter, EA
Ryder, D
Lanza, F
Nadali, G
Johnsen, H
Denning-Kendall, P
Thing-Mortensen, B
Silvestri, F
Testa, NG
机构
[1] Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England
[2] Christie Hosp NHS Trust, Manchester M20 4BX, Lancs, England
[3] St Anna Hosp, Inst Hematol, Ferrara, Italy
[4] Policlin Borgo Roma, Div & Cattedra Ematol, Verona, Italy
[5] Herlev Hosp, Dept Hematol, Herlev, Denmark
[6] Southmead Hosp, Dept Transplant Sci, Bristol, Avon, England
[7] Roskilde Univ, DK-4000 Roskilde, Denmark
[8] Univ Hosp, Udine, Italy
关键词
CD34(+) cells; positive selection; colony-forming cells; peripheral blood progenitor cells; umbilical cord blood; bone marrow;
D O I
10.1038/sj.bmt.1701789
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Primitive haemopoietic cells are required for studies in both the clinical and research fields and a number of systems have been developed to facilitate isolation of these haemopoietic cell populations. We have analysed the results from several European centres using positive selection of CD34(+) cells from haemopoietic tissues (n = 110), Four selection techniques including immunoaffinity columns (Ceprate LC), immunomagnetic beads (Dynabeads, Baxter Isolex 50) and submicroscopic magnetic beads (MACS) were used and the selected CD34(+) cells were assessed for purity, yield and enrichment of colony-forming cells (CFC), The mean purities for all samples ranged from 68.4-78.4% for MACS, 33.9-69.9% for Dynabeads, 46.9-66.8% for Ceprate LC and 43.2-65% for Baxter Isolex 50, Yields were variable,vith all techniques. On average CFC enrichment using the immunoaffinity columns was greater than that observed for the other systems. Some techniques appear to be problematic and may require further expertise to improve the results. Nevertheless, the study demonstrates that highly purified CD34(+) cells can be isolated from various haemopoietic sources, though yield and CFC enrichment varies significantly depending on the technique selected. This extends our previous report indicating that not all selection methods generate similar results and that there are differences in the purity, number and colony-forming ability of the cells recovered.
引用
收藏
页码:1191 / 1196
页数:6
相关论文
共 38 条
  • [1] BERENSON RJ, 1991, BLOOD, V77, P1717
  • [2] Megakaryocytic progenitors can be generated ex vivo and safely administered to autologous peripheral blood progenitor cell transplant recipients
    Bertolini, F
    Battaglia, M
    Pedrazzoli, P
    DaPrada, GA
    Lanza, A
    Soligo, D
    Caneva, L
    Sarina, B
    Murphy, S
    Thomas, T
    dellaCuna, GR
    [J]. BLOOD, 1997, 89 (08) : 2679 - 2688
  • [3] Effects of recombinant human thrombopoietin alone and in combination with erythropoietin and early-acting cytokines on human mobilized purified CD34(+) progenitor cells cultured in serum-depleted medium
    Birkmann, J
    Oez, S
    Smetak, M
    Kaiser, G
    Kappauf, H
    Gallmeier, WM
    [J]. STEM CELLS, 1997, 15 (01) : 18 - 32
  • [4] GROWTH-CHARACTERISTICS AND EXPANSION OF HUMAN UMBILICAL-CORD BLOOD AND ESTIMATION OF ITS POTENTIAL FOR TRANSPLANTATION IN ADULTS
    BROXMEYER, HE
    HANGOC, G
    COOPER, S
    RIBEIRO, RC
    GRAVES, V
    YODER, M
    WAGNER, J
    VADHANRAJ, S
    BENNINGER, L
    RUBINSTEIN, P
    BROUN, ER
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) : 4109 - 4113
  • [5] BRUGGER W, 1993, BLOOD, V81, P2579
  • [6] GM-CSF AND TNF-ALPHA COOPERATE IN THE GENERATION OF DENDRITIC LANGERHANS CELLS
    CAUX, C
    DEZUTTERDAMBUYANT, C
    SCHMITT, D
    BANCHEREAU, J
    [J]. NATURE, 1992, 360 (6401) : 258 - 261
  • [7] The purification of CD34(+) cells from human cord blood: Comparison of separation techniques and cytokine requirements for optimal growth of clonogenic progenitors
    Charbord, P
    Newton, I
    Voillat, L
    Schaal, JP
    Herve, P
    [J]. BRITISH JOURNAL OF HAEMATOLOGY, 1996, 94 (03) : 449 - 454
  • [8] CIVIN CI, 1984, J IMMUNOL, V133, P157
  • [9] Is in vitro expansion of human cord blood cells clinically relevant?
    Denning-Kendall, PA
    Nicol, A
    Horsley, H
    Donaldson, C
    Bradley, B
    Hows, JM
    [J]. BONE MARROW TRANSPLANTATION, 1998, 21 (03) : 225 - 232
  • [10] COMPARISON OF PURITY AND ENRICHMENT OF CD34(+) CELLS FROM BONE-MARROW, UMBILICAL-CORD AND PERIPHERAL-BLOOD (PRIMED FOR APHERESIS) USING 5 SEPARATION SYSTEMS
    DEWYNTER, EA
    COUTINHO, LH
    PEI, X
    MARSH, JCW
    HOWS, J
    LUFT, T
    TESTA, NG
    [J]. STEM CELLS, 1995, 13 (05) : 524 - 532