Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins

被引：100

作者：

Bermel, W

Bertini, I ^{[1
]}

Felli, IC

Kümmerle, R

Pierattelli, R

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy

[2] Univ Florence, Dept Chem, I-50121 Florence, Italy

[3] Bruker BioSpin GmbH, Rheinstetten, Germany

[4] Bruker BioSpin GmbH, Fallanden, Switzerland

来源：

JOURNAL OF MAGNETIC RESONANCE | 2006年 / 178卷 / 01期

关键词：

protonless NMR; C-13; NMR; C-13-direct detection; sequential assignment; protein structure;

D O I：

10.1016/j.jmr.2005.08.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments. (c) 2005 Elsevier Inc. All rights reserved.

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页码：56 / 64

页数：9

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