Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution

被引：78

作者：

Zha, DX

Wilensek, S

Hermes, M

Jaeger, KE

Reetz, MT ^{[1
]}

机构：

[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany

[2] Ruhr Univ Bochum, Lehrstuhl Mikroorganismen, D-44780 Bochum, Germany

来源：

CHEMICAL COMMUNICATIONS | 2001年 / 24期

关键词：

D O I：

10.1039/b109913m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The combination of error prone PCR at high mutation rate and DNA shuffling can be used to invert the direction of enantioselectivity of a lipase-catalyzed hydrolytic kinetic resolution involving a chiral ester.

引用

页码：2664 / 2665

页数：2

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