Patterned artificial juxtacrine stimulation of cells by covalently immobilized insulin

被引：37

作者：

Ito, Y ^{[1
]}

Kondo, S ^{[1
]}

Chen, GP ^{[1
]}

Imanishi, Y ^{[1
]}

机构：

[1] KYOTO UNIV,GRAD SCH ENGN,DEPT CHEM MAT,KYOTO 60601,JAPAN

来源：

FEBS LETTERS | 1997年 / 403卷 / 02期

关键词：

juxtacrine stimulation; patterned immobilization; tissue engineering; growth factor; insulin;

D O I：

10.1016/S0014-5793(97)00045-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin was immobilized on prescribed areas of poly(ethylene terephthalate) film by photolithography, Chinese hamster ovary cells overexpressing human insulin receptors were cultured on the pattern-immobilized film, Pattern-immobilized insulin did not enhance cell adhesion but transduced a signal to the cells through phosphorylation of tyrosine residues of signal proteins, In addition, only cells on immobilized insulin grew in medium containing no serum, The enhancement of cell growth was considered to be a consequence of signal transduction, This microprocessing technique is useful for investigating the effect of immobilized biosignal molecules and for constructing new tissue engineering materials. (C) 1997 Federation of European Biochemical Societies.

引用

页码：159 / 162

页数：4

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