myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis

被引:403
作者
Lorence, A
Chevone, BI
Mendes, P
Nessler, CL [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA
[2] Virginia Polytech Inst & State Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA
关键词
D O I
10.1104/pp.103.033936
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Two biosynthetic pathways for ascorbate (L-ascorbic acid [AsA]; vitamin C) in plants are presently known, the mannose/L-galactose pathway and an L-GalUA pathway. Here, we present molecular and biochemical evidence for a possible biosynthetic route using myo-inositol (MI) as the initial substrate. A MI oxygenase (MIOX) gene was identified in chromosome 4 (miox4) of Arabidopsis ecotype Columbia, and its enzymatic activity was confirmed in bacterially expressed recombinant protein. Miox4 was primarily expressed in flowers and leaves of wild-type Arabidopsis plants, tissues with a high concentration of AsA. Ascorbate levels increased 2- to 3-fold in homozygous Arabidopsis lines overexpressing the miox4 open reading frame, thus suggesting the role of MI in AsA biosynthesis and the potential for using this gene for the agronomic and nutritional enhancement of crops.
引用
收藏
页码:1200 / 1205
页数:6
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