Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis

被引:162
作者
Rottmann, WH
Meilan, R
Sheppard, LA
Brunner, AM
Skinner, JS
Ma, CP
Cheng, SP
Jouanin, L
Pilate, G
Strauss, SH
机构
[1] Oregon State Univ, Coll Forestry, Dept Forest Sci, Corvallis, OR 97331 USA
[2] INRA, Ameliorat Arbres Forestiers Stn, F-45160 Ardon, France
[3] INRA, Biol Cellulaire Lab, F-78026 Versailles, France
关键词
D O I
10.1046/j.1365-313x.2000.00734.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
PTLF, the Populus trichocarpa homolog of LEAFY (LFY) and FLORICAULA, was cloned to assess its function in a dioecious tree species. In situ hybridization studies showed that the gene was expressed most strongly in developing inflorescences. Expression was also seen in leaf primordia and very young leaves, most notably in apical vegetative buds near inflorescences, but also in seedlings. Although ectopic expression of the PTLF cDNA in Arabidopsis accelerated flowering, only one of the many tested transgenic lines of Populus flowered precociously, The majority of trees within a population of 3-year-old transgenic hybrid Populus lines with PTLF constitutively expressed showed few differences when compared to controls. However, phenotypic effects on growth rate and crown development, but not flowering, were seen in some trees with strong PTLF expression and became manifest only as the trees aged. Competence to respond to overexpression of LFY varied widely among Populus genotypes, giving consistent early flowering in only a single male P. tremula x P. tremuloides hybrid and causing gender change in another hybrid genotype. PTLF activity appears to be subject to regulation that does not affect heterologously expressed LFY, and is dependent upon tree maturation. Both genes provide tools for probing the mechanisms of delayed competence to flower in woody plants.
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收藏
页码:235 / 245
页数:11
相关论文
共 34 条
  • [11] Eckenwalder James E., 1996, P7
  • [12] FROHLICH MW, 2000, IN PRESS SYST BOT, V25
  • [13] An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus)
    Han, KH
    Meilan, R
    Ma, C
    Strauss, SH
    [J]. PLANT CELL REPORTS, 2000, 19 (03) : 315 - 320
  • [14] THE REGULATION OF FLOWERING IN ARABIDOPSIS-THALIANA - MERISTEMS, MORPHOGENESIS, AND MUTANTS
    HAUGHN, GW
    SCHULTZ, EA
    MARTINEZZAPATER, JM
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1995, 73 (07): : 959 - 981
  • [15] LEAF DEVELOPMENTAL AGE CONTROLS EXPRESSION OF GENES ENCODING ENZYMES OF CHLOROPHYLL AND HEME-BIOSYNTHESIS IN PEA (PISUM-SATIVUM L)
    HE, ZH
    LI, JM
    SUNDQVIST, C
    TIMKO, MP
    [J]. PLANT PHYSIOLOGY, 1994, 106 (02) : 537 - 546
  • [16] UNIFOLIATA regulates leaf and flower morphogenesis in pea
    Hofer, J
    Turner, L
    Hellens, R
    Ambrose, M
    Matthews, P
    Michael, A
    Ellis, N
    [J]. CURRENT BIOLOGY, 1997, 7 (08) : 581 - 587
  • [17] JEFFERSON RA, 1987, EMBO J, V6, P3901
  • [19] NFL, THE TOBACCO HOMOLOG OF FLORICAULA AND LEAFY, IS TRANSCRIPTIONALLY EXPRESSED IN BOTH VEGETATIVE AND FLORAL MERISTEMS
    KELLY, AJ
    BONNLANDER, MB
    MEEKSWAGNER, DR
    [J]. PLANT CELL, 1995, 7 (02) : 225 - 234
  • [20] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
    LAEMMLI, UK
    [J]. NATURE, 1970, 227 (5259) : 680 - +