Cytotoxicity of spin trapping compounds

被引：49

作者：

Haseloff, RF ^{[1
]}

Mertsch, K ^{[1
]}

Rohde, E ^{[1
]}

Baeger, I ^{[1
]}

Grigorev, IA ^{[1
]}

Blasig, IE ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST ORGAN CHEM,NOVOSIBIRSK 630090,RUSSIA

来源：

FEBS LETTERS | 1997年 / 418卷 / 1-2期

关键词：

cytotoxicity; spin trap; endothelial cell;

D O I：

10.1016/S0014-5793(97)01349-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spin trapping compounds are used frequently to detect free radicals released by cells. Their cytotoxicity has to be considered in order to prevent perturbations of normal cell growth and viability. Eleven spin traps (eight nitrones and three nitroso traps) have been tested for their effects on bovine aortic endothelial cells (toxicity range, 50% survival rate), The lowest cytotoxicity was found for 5,5-dimethylpyrroline-1-oxide and 2,2,4-trimethyl-2H-imidazole-1-oxide whereas nitrosobenzene and 2-methyl-2-nitrosopropane exerted the strongest cytotoxic effects. In addition, three nitronyl nitroxides mere tested, Their cytotoxicity was found to be dependent on substitution, and the toxic concentration of a lipophilic derivative was found to be more than two orders lower as compared to a hydrophilic derivative. The results of this study indicate that most spin traps can be used in cell cultures at customary (i.e. millimolar) concentrations; caution is recommended when nitroso spin traps are applied to cells. (C) 1997 Federation of European Biochemical Societies.

引用

页码：73 / 75

页数：3

共 23 条

[11] Biological spin trapping methodology .3. Octanol-water partition coefficients of spin-trapping compounds [J].

Janzen, EG ;

West, MS ;

Kotake, Y ;

DuBose, CM .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1996, 32 (03) :183-190

[12] STUDY OF REPRODUCIBILITY OF SPIN-TRAPPING RESULTS IN THE USE OF C-PHENYL-N-TERT-BUTYL NITRONE (PBN) FOR TRICHLOROMETHYL RADICAL DETECTION IN CCL4 METABOLISM BY RAT-LIVER MICROSOMAL DISPERSIONS - BIOLOGICAL SPIN-TRAPPING-I [J].

JANZEN, EG ;

POYER, JL ;

WEST, MS ;

CROSSLEY, C ;

MCCAY, PB .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1994, 29 (3-4) :189-205

[13] SPIN TRAPPING [J].

JANZEN, EG .

ACCOUNTS OF CHEMICAL RESEARCH, 1971, 4 (01) :31-&

[14] DNA STRAND BREAKAGE BY HYDROXYPHENYL RADICALS GENERATED FROM MUTAGENIC DIAZOQUINONE COMPOUNDS [J].

KATO, T ;

KOJIMA, K ;

HIRAMOTO, K ;

KIKUGAWA, K .

MUTATION RESEARCH, 1992, 268 (01) :105-114

[15] Superoxide anion and endothelial regulation of arterial tone [J].

Katusic, ZS .

FREE RADICAL BIOLOGY AND MEDICINE, 1996, 20 (03) :443-448

[16] SPIN-TRAPPING USING 2,2-DIMETHYL-2H-IMIDAZOLE-1-OXIDES [J].

KLAUSCHENZ, E ;

HASELOFF, RF ;

VOLODARSKII, LB ;

BLASIG, IE .

FREE RADICAL RESEARCH, 1994, 20 (02) :103-111

[17] VASODILATORY AND TOXIC EFFECTS OF SPIN TRAPS ON AEROBIC CARDIAC-FUNCTION [J].

KONOREV, EA ;

BAKER, JE ;

JOSEPH, J ;

KALYANARAMAN, B .

FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (02) :127-137

[18] PROTEASE-CLEAVED IRON-TRANSFERRIN AUGMENTS OXIDANT-MEDIATED ENDOTHELIAL-CELL INJURY VIA HYDROXYL RADICAL FORMATION [J].

MILLER, RA ;

BRITIGAN, BE .

JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (06) :2491-2500

[19]

Siems W E, 1985, Z Med Lab Diagn, V26, P232

[20] In vivo trapping of nitric oxide in the brain of neonatal rats treated with the HIV-1 envelope protein gp 120: Protective effects of alpha-phenyl-tert-butylnitrone [J].

Tabatabaie, T ;

Stewart, C ;

Pye, Q ;

Kotake, Y ;

Floyd, RA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (02) :386-390

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