Cyclosporin A as a model antigen: immunochemical and structural studies

被引：8

作者：

Altschuh, D ^{[1
]}

机构：

[1] Univ Strasbourg 1, Ecole Super Biotechnol Strasbourg, CNRS, UMR Biotechnol Interact Mol 7100, F-67400 Illkirch Graffenstaden, France

来源：

JOURNAL OF MOLECULAR RECOGNITION | 2002年 / 15卷 / 05期

关键词：

cyclosporin; Fab-antigen complex; crystallographic analysis; antigen-antibody affinity; Biacore; competition ELISA; structure-activity relationship;

D O I：

10.1002/jmr.588

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inummosuppressant drug cyclosporin (Cs) A is a cyclic undecapeptide which has been used as a model antigen because structural information and a large number of analogs, modified at each of its 11 positions, were available. This review summarizes immunochemical and crystallographic studies of the interaction between the Fab of monoclonal antibody R45-45-11 and Cs. Three points are discussed: (1) the different conformations of CsA and the question of its biologically active form; (2) the Fab-CsA recognition mechanism; and (3) the relationship between structure and binding properties of CsA analogs. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：277 / 285

页数：11

共 51 条

[1] A CONFORMATION OF CYCLOSPORINE-A IN AQUEOUS ENVIRONMENT REVEALED BY THE X-RAY STRUCTURE OF A CYCLOSPORINE-FAB COMPLEX [J].

ALTSCHUH, D ;

VIX, O ;

REES, B ;

THIERRY, JC .

SCIENCE, 1992, 256 (5053) :92-94

[2] CONFORMATIONAL POLYMORPHISM OF CYCLOSPORINE-A [J].

ALTSCHUH, D ;

BRAUN, W ;

KALLEN, J ;

MIKOL, V ;

SPITZFADEN, C ;

THIERRY, LC ;

VIX, O ;

WALKINSHAW, MD ;

WUTHRICH, K .

STRUCTURE, 1994, 2 (10) :963-972

[3] Anatomy of hot spots in protein interfaces [J].

Bogan, AA ;

Thorn, KS .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 280 (01) :1-9

[4] 3-DIMENSIONAL STRUCTURE AND ACTIONS OF IMMUNOSUPPRESSANTS AND THEIR IMMUNOPHILINS [J].

BRAUN, W ;

KALLEN, J ;

MIKOL, V ;

WALKINSHAW, MD ;

WUTHRICH, K .

FASEB JOURNAL, 1995, 9 (01) :63-72

[5] HYDROPHOBIC BONDING AND ACCESSIBLE SURFACE-AREA IN PROTEINS [J].

CHOTHIA, C .

NATURE, 1974, 248 (5446) :338-339

[6] A mutational analysis of binding interactions in an antigen-antibody protein-protein complex [J].

Dall'Acqua, W ;

Goldman, ER ;

Lin, WH ;

Teng, C ;

Tsuchiya, D ;

Li, HM ;

Ysern, X ;

Braden, BC ;

Li, YL ;

Smith-Gill, SJ ;

Mariuzza, RA .

BIOCHEMISTRY, 1998, 37 (22) :7981-7991

[7] Unraveling hot spots in binding interfaces: progress and challenges [J].

DeLano, WL .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2002, 12 (01) :14-20

[8] Effects of substitutions in the binding surface of an antibody on antigen affinity [J].

Dougan, DA ;

Malby, RL ;

Gruen, LC ;

Kortt, AA ;

Hudson, PJ .

PROTEIN ENGINEERING, 1998, 11 (01) :65-74

[9] NMR-STUDIES OF [U-C-13]CYCLOSPORIN-A BOUND TO CYCLOPHILIN - BOUND CONFORMATION AND PORTIONS OF CYCLOSPORINE INVOLVED IN BINDING [J].

FESIK, SW ;

GAMPE, RT ;

EATON, HL ;

GEMMECKER, G ;

OLEJNICZAK, ET ;

NERI, P ;

HOLZMAN, TF ;

EGAN, DA ;

EDALJI, R ;

SIMMER, R ;

HELFRICH, R ;

HOCHLOWSKI, J ;

JACKSON, M .

BIOCHEMISTRY, 1991, 30 (26) :6574-6583

[10] The three-dimensional structure of a complex of a murine Fab (NC10.14) with a potent sweetener (NC174): An illustration of structural diversity in antigen recognition by immunoglobulins [J].

Guddat, LW ;

Shan, L ;

Broomell, C ;

Ramsland, PA ;

Fan, ZC ;

Anchin, JM ;

Linthicum, DS ;

Edmundson, AB .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 302 (04) :853-872

← 1 2 3 4 5 6 →