Cyclosporin A as a model antigen: immunochemical and structural studies

被引：8

作者：

Altschuh, D ^{[1
]}

机构：

[1] Univ Strasbourg 1, Ecole Super Biotechnol Strasbourg, CNRS, UMR Biotechnol Interact Mol 7100, F-67400 Illkirch Graffenstaden, France

来源：

JOURNAL OF MOLECULAR RECOGNITION | 2002年 / 15卷 / 05期

关键词：

cyclosporin; Fab-antigen complex; crystallographic analysis; antigen-antibody affinity; Biacore; competition ELISA; structure-activity relationship;

D O I：

10.1002/jmr.588

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inummosuppressant drug cyclosporin (Cs) A is a cyclic undecapeptide which has been used as a model antigen because structural information and a large number of analogs, modified at each of its 11 positions, were available. This review summarizes immunochemical and crystallographic studies of the interaction between the Fab of monoclonal antibody R45-45-11 and Cs. Three points are discussed: (1) the different conformations of CsA and the question of its biologically active form; (2) the Fab-CsA recognition mechanism; and (3) the relationship between structure and binding properties of CsA analogs. Copyright (C) 2002 John Wiley Sons, Ltd.

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页码：277 / 285

页数：11

相关论文

共 51 条

[31]

QUESNIAUX V, 1986, PROG ALLERGY, V38, P108

[32] FINE SPECIFICITY AND CROSS-REACTIVITY OF MONOCLONAL-ANTIBODIES TO CYCLOSPORINE [J].

QUESNIAUX, VFJ ;

TEES, R ;

SCHREIER, MH ;

WENGER, RM ;

VANREGENMORTEL, MHV .

MOLECULAR IMMUNOLOGY, 1987, 24 (11) :1159-1168

[33] Incorporation of long CDR3s into V domains: Implications for the structural evolution of the antibody-combining site [J].

Ramsland, PA ;

Kaushik, A ;

Marchalonis, JJ ;

Edmundson, AB .

EXPERIMENTAL AND CLINICAL IMMUNOGENETICS, 2001, 18 (04) :176-198

[34] STRUCTURE-ACTIVITY-RELATIONSHIPS FOR THE INTERACTION BETWEEN CYCLOSPORINE-A DERIVATIVES AND THE FAB FRAGMENT OF A MONOCLONAL-ANTIBODY [J].

RAUFFER, N ;

ZEDERLUTZ, G ;

WENGER, R ;

VANREGENMORTEL, MHV ;

ALTSCHUH, D .

MOLECULAR IMMUNOLOGY, 1994, 31 (12) :913-922

[35] Crystal structure of a neutralizing human IgG against HIV-1: A template for vaccine design [J].

Saphire, EO ;

Parren, PWHI ;

Pantophlet, R ;

Zwick, MB ;

Morris, GM ;

Rudd, PM ;

Dwek, RA ;

Stanfield, RL ;

Burton, DR ;

Wilson, IA .

SCIENCE, 2001, 293 (5532) :1155-1159

[36] ANALYSIS OF THE STRUCTURAL DIVERSITY OF MONOCLONAL-ANTIBODIES TO CYCLOSPORINE [J].

SCHMITTER, D ;

POCH, O ;

ZEDER, G ;

HEINRICH, GF ;

KOCHER, HP ;

QUESNIAUX, VFJ ;

VANREGENMORTEL, MHV .

MOLECULAR IMMUNOLOGY, 1990, 27 (10) :1029-1038

[37] DETERMINATION OF THE NMR SOLUTION STRUCTURE OF THE CYCLOPHILIN-A - CYCLOSPORINE-A COMPLEX [J].

SPITZFADEN, C ;

BRAUN, W ;

WIDER, G ;

WIDMER, H ;

WUTHRICH, K .

JOURNAL OF BIOMOLECULAR NMR, 1994, 4 (04) :463-482

[38] Dual conformations for the HIV-1 gp120 V3 loop in complexes with different neutralizing Fabs [J].

Stanfield, RL ;

Cabezas, E ;

Satterthwait, AC ;

Stura, EA ;

Profy, AT ;

Wilson, IA .

STRUCTURE, 1999, 7 (02) :131-142

[39]

STEIN R L, 1991, Current Biology, V1, P234, DOI 10.1016/0960-9822(91)90067-7

[40] Estimation of the hydrophobic effect in an antigen-antibody protein-protein interface [J].

Sundberg, EJ ;

Urrutia, M ;

Braden, BC ;

Isern, J ;

Tsuchiya, D ;

Fields, BA ;

Malchiodi, EL ;

Tormo, J ;

Schwarz, FP ;

Mariuzza, RA .

BIOCHEMISTRY, 2000, 39 (50) :15375-15387

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