Trigonopsis variabilis D-amino acid oxidase:: Control of protein quality and opportunities for biocatalysis through production in Escherichia coli

被引：22

作者：

Dib, Iskandar

Stanzer, Damir

Nidetzky, Bernd

机构：

[1] Graz Univ Technol, Inst Biotechnol & Biochem Engn, A-8010 Graz, Austria

[2] Res Ctr Appl Biocatalysis, A-8010 Graz, Austria

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 01期

关键词：

D O I：

10.1128/AEM.01569-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Trigonopsis variabilis D-amino acid oxidase accounts for 35% of Escherichia coli protein when added D-methionine suppresses the toxic activity of the recombinant product. Permeabilized E. coli cells are reusable and stabilized enzyme preparations. The purified oxidase lacks the microheterogeneity of the natural enzyme. Oriented immobilization of a chimeric oxidase maintains 80% of the original activity in microparticle-bound enzymes.

引用

收藏

页码：331 / 333

页数：3

相关论文

共 18 条

[1] Engineering the D-amino acid oxidase from Trigonopsis variabilis to facilitate its overproduction in Escherichia coli and its downstream processing by tailor-made metal chelate supports [J].

Alonso, J ;

Barredo, JL ;

Armisén, P ;

Díez, B ;

Salto, F ;

Guisán, JM ;

García, JL ;

Cortés, E .

ENZYME AND MICROBIAL TECHNOLOGY, 1999, 25 (1-2) :88-95

[2]

Buchholz K, 2005, BIOCATALYSTS ENZYME, P381

[3] Coexpression of Vitreoscilla hemoglobin reduces the toxic effect of expression of D-amino acid oxidase in E. coli [J].

Chien, LJ ;

Wu, JM ;

Kuan, IC ;

Lee, CK .

BIOTECHNOLOGY PROGRESS, 2004, 20 (05) :1359-1365

[4] Thermal inactivation of D-amino acid oxidase from Trigonopsis variabilis occurs via three parallel paths of irreversible denaturation [J].

Dib, Iskandar ;

Slavica, Anita ;

Riethorst, Waander ;

Nidetzky, Bernd .

BIOTECHNOLOGY AND BIOENGINEERING, 2006, 94 (04) :645-654

[5]

Fantinato S., 2001, ENZYME MICROB TECHNO, V29, P407

[6] High-level expression of Trigonopsis variabilis D-amino acid oxidase in Escherichia coli using lactose as inducer [J].

Hwang, TS ;

Fu, HM ;

Lin, LL ;

Hsu, WH .

BIOTECHNOLOGY LETTERS, 2000, 22 (08) :655-658

[7] A conserved aspartate is essential for FAD binding and catalysis in the D-amino acid oxidase from Trigonopsis variabilis [J].

Ju, SS ;

Lin, LL ;

Wang, WC ;

Hsu, WH .

FEBS LETTERS, 1998, 436 (01) :119-122

[8]

Liese A., 2006, IND BIOTRANSFORMATIO, V2nd

[9] Expression of Trigonopsis variabilis D-amino acid oxidase gene in Escherichia coli and characterization of its inactive mutants [J].

Lin, LL ;

Chien, HR ;

Wang, WC ;

Hwang, TS ;

Fu, HM ;

Hsu, WH .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (07) :482-491

[10] Recent trends in enzymatic conversion of cephalosporin C to 7-Aminocephalosporanic acid (7-ACA) [J].

Parmar, A ;

Kumar, H ;

Marwaha, SS ;

Kennedy, JF .

CRITICAL REVIEWS IN BIOTECHNOLOGY, 1998, 18 (01) :1-12