Sequence analysis of the nucleoprotein gene of Asian lineage peste des petits ruminants vaccine virus

被引：24

作者：

Muthuchelvan, D.

Sanyal, A.

Balamurugan, V.

Dhar, P.

Bandyopadhyay, S. K. ^{[1
]}

机构：

[1] Anim Husb Commissioner, New Delhi, India

[2] Cent Inst Fisheries Technol, Cochin, Kerala, India

[3] Project Directorate Foot & Mouth Dis, Naini Tal, India

[4] Indian Vet Res Inst, Div Virol, Naini Tal, India

来源：

VETERINARY RESEARCH COMMUNICATIONS | 2006年 / 30卷 / 08期

关键词：

Morbillivirus; peste-des-petits ruminants virus; vaccine virus; N gene; nucleotide sequence; amino acid sequence;

D O I：

10.1007/s11259-006-3407-0

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The complete nucleotide sequence of the nucleocapsid (N) protein of the peste-des-petits ruminants vaccine virus (PPRV Sungri/96) belonging to the Asian lineage was determined. The gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino acids. The PPRV Sungri/96 N gene has a nucleotide homology of 92% for PPRV Nigeria 75/1 to 55.5% for canine distemper virus. At amino acid level the homology was 94.1% with PPRV Nigeria 75/1, while with other morbilliviruses, PPRV Sungri/96 had only 71.4-64.9% amino acid identity. The phosphorylation prediction reveals eight conserved sites across morbilliviruses, whereas in the C-terminal portion of the protein the sites are not conserved. Phylogenetic analysis of different N proteins of morbilliviruses revealed five well-defined clusters as observed previously. To the best of our knowledge this is the first report describing the nucleocapsid gene sequence of PPRV Indian isolate.

引用

页码：957 / 963

页数：7

共 21 条

[11] The matrix protein gene sequence analysis reveals close relationship between peste des petits ruminants virus (PPRV) and dolphin morbillivirus [J].