Phosphatidylethanolamine has an essential role in Saccharomyces cerevisiae that is independent of its ability to form hexagonal phase structures

被引:104
作者
Storey, MK
Clay, KL
Kutateladze, T
Murphy, RC
Overduin, M
Voelker, DR [1 ]
机构
[1] Natl Jewish Med & Res Ctr, Dept Med, Denver, CO 80206 USA
[2] Natl Jewish Med & Res Ctr, Dept Pediat, Denver, CO 80206 USA
[3] Natl Jewish Med & Res Ctr, Cell Biol Program, Denver, CO 80206 USA
[4] Univ Colorado, Hlth Sci Ctr, Dept Pharmacol, Denver, CO USA
关键词
D O I
10.1074/jbc.M109043200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two yeast enzymes, Psd1p and Psd2p, catalyze the decarboxylation of phosphatidylserine to produce phosphatidylethanolamine (PtdEtn). Mitochondrial Psd1p provides similar to 90% of total cellular phosphatidylserine decarboxylase activity. When the PSD1 gene is deleted, the resultant strain (psd1 Delta) grows normally at 30 degreesC in glucose and in the absence of exogenous choline or ethanolamine. However, at elevated temperature (37 degreesC) or on the nonfermentable carbon source lactate, the growth of psd1 Delta strains is minimal without ethanolamine supplementation. The reduced growth and viability correlate with a PtdEtn content below 4% of total phospholipid. These results suggest that there is a critical level of PtdEtn required to support growth. This theory is supported by growth data revealing that a psd1 Delta psd2 Delta dpl1 Delta strain can only grow in the presence of ethanolamine. In contrast, a psd1 Delta psd2 Delta strain, which makes low levels of PtdEtn from sphingolipid breakdown, can be rescued by ethanolamine, choline, or the ethanolamine analogue propanolamine. psd1 Delta psd2 Delta cells grown in 2 mM propanolamine accumulate a novel lipid, which was determined by mass spectrometry to be phosphatidylpropanolamine (PtdPrn). PtdPrn can comprise up to 40% of the total phospholipid content in supplemented cells at the expense of phosphatidylcholine and PtdEtn. The absolute level of PtdEtn required for growth when PtdPrn is present appears to be 1% of the total phospholipid content. The essential function of the PtdEtn in the presence of propanolamine does not appear to be the formation of hexagonal phase lipid, insofar as PtdPrn readily forms hexagonal phase structures detectable by P-31 NMR.
引用
收藏
页码:48539 / 48548
页数:10
相关论文
共 46 条
[41]  
TROTTER PJ, 1993, J BIOL CHEM, V268, P21416
[42]   Membrane phospholipid dynamics during cytokinesis: regulation of actin filament assembly by redistribution of membrane surface phospholipid [J].
Umeda, M ;
Emoto, K .
CHEMISTRY AND PHYSICS OF LIPIDS, 1999, 101 (01) :81-91
[43]  
VERKLEIJ AJ, 1984, CIBA F SYMP, V103, P45
[44]   THE LIPIDIC PARTICLE AS AN INTERMEDIATE STRUCTURE IN MEMBRANE-FUSION PROCESSES AND BILAYER TO HEXAGONAL HII TRANSITIONS [J].
VERKLEIJ, AJ ;
VANECHTELD, CJA ;
GERRITSEN, WJ ;
CULLIS, PR ;
DEKRUIJFF, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 600 (03) :620-624
[45]   Evidence for an intrinsic toxicity of phosphatidylcholine to Sec14p-dependent protein transport from the yeast Golgi complex [J].
Xie, ZH ;
Fang, M ;
Bankaitis, VA .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (04) :1117-1129
[46]   LIPID REGULATION OF CELL-MEMBRANE STRUCTURE AND FUNCTION [J].
YEAGLE, PL .
FASEB JOURNAL, 1989, 3 (07) :1833-1842