DNA sequencing by delayed extraction matrix-assisted laser desorption/ionization time of flight mass spectrometry

被引：108

作者：

Roskey, MT ^{[1
]}

Juhasz, P ^{[1
]}

Smirnov, IP ^{[1
]}

Takach, EJ ^{[1
]}

Martin, SA ^{[1
]}

Haff, LA ^{[1
]}

机构：

[1] PERSEPT BIOSYST,FRAMINGHAM,MA 01701

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 10期

关键词：

D O I：

10.1073/pnas.93.10.4724

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Matrix-assisted laser desorption/ionization (MALDI) time of flight mass spectrometry was used to detect and order DNA fragments generated by Sanger dideoxy cycle sequencing. This was accomplished by improving the sensitivity and resolution of the MALDI method using a delayed ion extraction technique (DE-MALDI). The cycle sequencing chemistry was optimized to produce as much as 100 fmol of each specific dideoxy terminated fragment, generated from extension of a 13-base primer annealed on 40- and 50-base templates, Analysis of the resultant sequencing mixture by DE-MALDI identified the appropriate termination products. The technique provides a new non-gel-based method to sequence DNA which may ultimately have considerable speed advantages over traditional methodologies.

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页码：4724 / 4729

页数：6

相关论文

共 22 条

[1] A NONRADIOACTIVE AUTOMATED-METHOD FOR DNA-SEQUENCE DETERMINATION [J].

ANSORGE, W ;

SPROAT, BS ;

STEGEMANN, J ;

SCHWAGER, C .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1986, 13 (06) :315-323

[2] MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE [J].

BAI, J ;

LIU, YH ;

LUBMAN, DM ;

SIEMIENIAK, D .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (09) :687-691

[3]

BROWN JS, 1995, ANAL CHEM, V67, P1998

[4] NOVEL NON-TEMPLATED NUCLEOTIDE ADDITION-REACTIONS CATALYZED BY PROCARYOTIC AND EUKARYOTIC DNA-POLYMERASES [J].

CLARK, JM .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9677-9686

[5] IMPROVING THE RESOLUTION OF MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY BY EXPLOITING THE CORRELATION BETWEEN ION POSITION AND VELOCITY [J].

COLBY, SM ;

KING, TB ;

REILLY, JP .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (11) :865-868

[6] THE ANALYSIS OF MOCK DNA-SEQUENCING REACTIONS USING MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY [J].

FITZGERALD, MC ;

ZHU, L ;

SMITH, LM .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (10) :895-897

[7] ANTISENSE DNA OLIGONUCLEOTIDES .2. THE USE OF MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY FOR THE SEQUENCE VERIFICATION OF METHYLPHOSPHONATE OLIGODEOXYRIBONUCLEOTIDES [J].

KEOUGH, T ;

BAKER, TR ;

DOBSON, RLM ;

LACEY, MP ;

RILEY, TA ;

HASSELFIELD, JA ;

HESSELBERTH, PE .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (03) :195-200

[8] 7-DEAZA PURINE-BASES OFFER A HIGHER ION STABILITY IN THE ANALYSIS OF DNA BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY [J].

KIRPEKAR, F ;

NORDHOFF, E ;

KRISTIANSEN, K ;

ROEPSTORFF, P ;

HAHNER, S ;

HILLENKAMP, F .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (06) :525-531

[9] ION STABILITY OF NUCLEIC-ACIDS IN INFRARED MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY [J].

NORDHOFF, E ;

CRAMER, R ;

KARAS, M ;

HILLENKAMP, F ;

KIRPEKAR, F ;

KRISTIANSEN, K ;

ROEPSTORFF, P .

NUCLEIC ACIDS RESEARCH, 1993, 21 (15) :3347-3357

[10] MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY - A POWERFUL TOOL FOR THE MASS AND SEQUENCE-ANALYSIS OF NATURAL AND MODIFIED OLIGONUCLEOTIDES [J].

PIELES, U ;

ZURCHER, W ;

SCHAR, M ;

MOSER, HE .

NUCLEIC ACIDS RESEARCH, 1993, 21 (14) :3191-3196