miR-21 mediates hematopoietic suppression in MDS by activating TGF-β signaling

被引：116

作者：

Bhagat, Tushar D. ^{[1
]}

Zhou, Li ^{[1
]}

Sokol, Lubomir ^{[2
]}

Kessel, Rachel ^{[1
]}

Caceres, Gisela ^{[2
]}

Gundabolu, Krishna ^{[1
]}

Tamari, Roni ^{[1
]}

Gordon, Shanisha ^{[1
]}

Mantzaris, Ioannis ^{[1
]}

Jodlowski, Tomasz ^{[1
]}

Yu, Yiting ^{[1
]}

Jing, Xiaohong ^{[1
]}

Polineni, Rahul ^{[1
]}

Bhatia, Kavi ^{[1
]}

Pellagatti, Andrea ^{[3
]}

Boultwood, Jacqueline ^{[3
]}

Kambhampati, Suman ^{[4
]}

Steidl, Ulrich ^{[1
]}

Stein, Cy ^{[1
]}

Ju, Wenjun ^{[5
]}

Liu, Gang ^{[6
]}

Kenny, Paraic ^{[1
]}

List, Alan ^{[2
]}

Bitzer, Markus ^{[1
,5
]}

Verma, Amit ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Bronx, NY 10467 USA

[2] Univ S Florida, H Lee Moffitt Canc Ctr, Tampa, FL 33682 USA

[3] John Radcliffe Hosp, NDCLS, LLR Mol Haematol Unit, Oxford OX3 9DU, England

[4] Univ Kansas, Med Ctr, VA Med Ctr, Kansas City, MO USA

[5] Univ Michigan, Ann Arbor, MI 48105 USA

[6] Univ Alabama Birmingham, Birmingham, AL USA

来源：

BLOOD | 2013年 / 121卷 / 15期

基金：

美国国家卫生研究院;

关键词：

GROWTH-FACTOR-BETA; MYELODYSPLASTIC SYNDROMES; EXPRESSION; APOPTOSIS; CELLS; CYTOKINES; PATHWAY; CANCER; MYELOFIBROSIS; RECEPTORS;

D O I：

10.1182/blood-2011-12-397067

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Myelodysplastic syndromes (MDS) are characterized by ineffective hematopoiesis that leads to peripheral cytopenias. We observed that SMAD7, a negative regulator of transforming growth factor-beta eta (TGF-beta) receptor-I kinase, is markedly reduced in MDS and leads to ineffective hematopoiesis by overactivation of TGF-beta signaling. To determine the cause of SMAD7 reduction inMDS, we analyzed the 3'UTR of the gene and determined that it contains a highly conserved putative binding site for microRNA-21. We observed significantly elevated levels of miR-21 in MDS marrow samples when compared with agematched controls. miR-21 was shownto directly bind to the 3'UTRofSMAD7 and reduce its expression in hematopoietic cells. Next, we tested the role of miR-21 in regulating TGF-beta signaling in a TGF-beta-overexpressing transgenic mouse model that develops progressive anemia and dysplasia and thus serves as a model of human bone marrow failure. Treatment with a chemically modified miR-21 inhibitor led to significant increases in hematocrit and led to an increase in SMAD7 expression in vivo. Inhibition of miR-21 also led to an increase in erythroid colony formation from primary MDS bone marrow progenitors, demonstrating its ability in stimulating hematopoiesis in vitro. Taken together, these studies demonstrate the role ofmiR-21 in regulating overactivated TGF-beta signaling in MDS.

引用

页码：2875 / 2881

页数：7

共 34 条

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