Mechanisms of secretion of ATP from cortical astrocytes triggered by uridine triphosphate

被引：28

作者：

Abdipranoto, A

Liu, GJ

Werry, EL

Bennett, MR ^{[1
]}

机构：

[1] Univ Sydney, Dept Physiol, Neurobiol Lab, Sydney, NSW 2006, Australia

[2] Univ Sydney, Inst Biomed Res, Sydney, NSW 2006, Australia

来源：

NEUROREPORT | 2003年 / 14卷 / 17期

关键词：

astrocytes; cortex; purines; secretion;

D O I：

10.1097/00001756-200312020-00009

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The mechanisms involved in autocrine ATP release from cultured astrocytes isolated from the rat cortex were investigated using an online bioluminescence technique. Astrocytes released ATP in response to application of 10 muM uridine triphosphate, which was blocked by the non-specific purinergic receptor antagonist suramin. Intracellular pathways of the uridine triphosphate-stimulated ATP release were seen to involve inositol triphosphate and calcium with the assistance of the Golgi-complex and cytoskeleton as the release was inhibited by phospholipase C antagonist lithium, endoplasmic reticulum calcium-dependent ATPase inhibitor thapsigargin, F-actin interruptor cytochalasin D and Golgi-complex interruptor brefeldin A. The uridine triphosphate-stimulated ATP release was also potently blocked by exocytosis inhibitor botulinum toxin A and anion transporter blockers furosemide and glibenclamide. These results suggest that calcium-dependent exocytosis and transportation via anion transporters are the predominant secretion mechanisms for uridine triphosphate-stimulated ATP release from cortical astrocytes.

引用

页码：2177 / 2181

页数：5

共 28 条

[1] Rat astroglial P2Z (P2X(7)) receptors regulate intracellular calcium and purine release [J].