Effects of brassinazole, an inhibitor of brassinosteroid biosynthesis, on light- and dark-grown Chlorella vulgaris

被引:28
作者
Bajguz, A
Asami, T
机构
[1] Univ Bialystok, Inst Biol, PL-15950 Bialystok, Poland
[2] RIKEN, Wako, Saitama 3510198, Japan
关键词
brassinazole; brassinolide; Chlorella; clomazone; mevinolin; non-mevalonate pathway;
D O I
10.1007/s00425-003-1170-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Treatment of cultured Chlorella vulgaris Beijerinck cells with 0.1-10 muM brassinazole (Brz2001), an inhibitor of brassinosteroid (BR) biosynthesis, inhibits their growth during the first 48 h of cultivation in the light. This inhibition is prevented by the co-application of BR. This result suggests that the presence of endogenous BRs during the initial steps of the C. vulgaris cell cycle is indispensable for their normal growth in the light. In darkness, a treatment with 10 nM brassinolide (BL) promotes growth through the first 24 h of culture, but during the following 24 h the cells undergo complete stagnation. Treatment of dark-grown cells with either Brz2001 alone, or a mixture of 10 nM BL and 0.1/10 muM Brz2001, also stimulates their growth. The effects of treatment with 10 nM BL mixed with 0.1-10 muM of a mevalonate-pathway inhibitor (mevinolin), or a non-mevalonate-pathway inhibitor (clomazone), were also investigated. Mevinolin at these concentrations did not inhibit growth of C. vulgaris; however, clomazone did. Addition of BL overcame the inhibition. These results suggest that the mevalonate pathway does not function in C. vulgaris, and that the non-mevalonate pathway for isopentenyl diphosphate biosynthesis is responsible for the synthesis of one of the primary precursors in BR biosynthesis.
引用
收藏
页码:869 / 877
页数:9
相关论文
共 43 条
[1]   Brassinosteroid biosynthesis inhibitors [J].
Asami, T ;
Yoshida, S .
TRENDS IN PLANT SCIENCE, 1999, 4 (09) :348-353
[2]   Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in Planta [J].
Asami, T ;
Mizutani, M ;
Fujioka, S ;
Goda, H ;
Min, YK ;
Shimada, Y ;
Nakano, T ;
Takatsuto, S ;
Matsuyama, T ;
Nagata, N ;
Sakata, K ;
Yoshida, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) :25687-25691
[3]   Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor [J].
Asami, T ;
Min, YK ;
Nagata, N ;
Yamagishi, K ;
Takatsuto, S ;
Fujioka, S ;
Murofushi, N ;
Yamaguchi, I ;
Yoshida, S .
PLANT PHYSIOLOGY, 2000, 123 (01) :93-99
[4]   Physiological and biochemical role of brassinosteroids and their structure-activity relationship in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae) [J].
Bajguz, A ;
Czerpak, R .
JOURNAL OF PLANT GROWTH REGULATION, 1998, 17 (03) :131-139
[5]   The chemical characteristic and distribution of brassinosteroids in plants [J].
Bajguz, A ;
Tretyn, A .
PHYTOCHEMISTRY, 2003, 62 (07) :1027-1046
[6]   Plants steroid hormones, brassinosteroids: Current highlights of molecular aspects on their synthesis/metabolism, transport, perception and response [J].
Bishop, GJ ;
Yokota, T .
PLANT AND CELL PHYSIOLOGY, 2001, 42 (02) :114-120
[7]  
BUSCHMANN E, 1987, LIEBIGS ANN CHEM, P349
[8]   The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis [J].
Choe, S ;
Dilkes, BP ;
Gregory, BD ;
Ross, AS ;
Yuan, H ;
Noguchi, T ;
Fujioka, S ;
Takatsuto, S ;
Tanaka, A ;
Yoshida, S ;
Tax, FE ;
Feldmann, KA .
PLANT PHYSIOLOGY, 1999, 119 (03) :897-907
[9]   The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22α-hydroxylation steps in brassinosteroid biosynthesis [J].
Choe, SW ;
Dilkes, BP ;
Fujioka, S ;
Takatsuto, S ;
Sakurai, A ;
Feldmann, KA .
PLANT CELL, 1998, 10 (02) :231-243
[10]   Brassinosteroids: Essential regulators of plant growth and development [J].
Clouse, SD ;
Sasse, JM .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :427-451