Cryocrystallography with oil - an old idea revived

被引：54

作者：

Riboldi-Tunnicliffe, A ^{[1
]}

Hilgenfeld, R ^{[1
]}

机构：

[1] Inst Mol Biotechnol, Dept Struct Biol & Crystallog, D-07745 Jena, Germany

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 1999年 / 32卷

关键词：

D O I：

10.1107/S0021889899008584

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method is described that permits the cooling of protein crystals without the use of conventional cryoprotective agents. Covering the crystal completely with highly liquid dry paraffin oil (Merck) prevents ice formation. It is suggested that this method, because of its simplicity, be tried as a first choice in cryocrystallography, since it does not require the growth or soaking of crystals in solvents which could disturb the packing by diffusing into the crystal.

引用

页码：1003 / 1005

页数：3

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