Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro

被引：18

作者：

Yu, Congtao ^{[1
]}

Dai, Lifen ^{[2
]}

Ma, Zhaoxia ^{[1
]}

Zhao, Hongbin ^{[3
]}

Yuan, Yong ^{[2
]}

Zhang, Yunfeng ^{[4
]}

Bao, Pengfei ^{[1
]}

Su, Yanfang ^{[1
]}

Ma, Daiping ^{[1
]}

Liu, Change ^{[1
]}

Wu, Xingfei ^{[1
]}

Liu, Jinxue ^{[1
]}

Li, Yanjiao ^{[1
]}

Wang, Bing ^{[5
]}

Hu, Min ^{[1
]}

机构：

[1] Kunming Univ, Yunnan Key Lab Basic Res Bone & Joint Dis, Kunming 650214, Yunnan, Peoples R China

[2] Kunming Med Univ, Affiliated Hosp 2, Kunming 650500, Yunnan, Peoples R China

[3] Kunming Univ Sci & Technol, Peoples Hosp Yunnan Prov 1, Affiliated Hosp, Dept Emergency Surg, Kunming 650032, Yunnan, Peoples R China

[4] Disabled Rehabil Ctr Yunnan Prov, Bone & Joint Rehabil Dept, Kunming 650032, Yunnan, Peoples R China

[5] Kunming Med Univ, Affiliated Hosp 1, Kunming 650031, Yunnan, Peoples R China

来源：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2019年 / 19卷 / 1期

关键词：

Osteoking; Mesenchymal stem cells; Osteogenic differentiation; Adipogenic differentiation; Bone disease; MSCS;

D O I：

10.1186/s12906-019-2435-6

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

BackgroundBone damage is a condition that affects the quality of life of patients. Mesenchymal stem cells (MSCs) are important for bone repair. Osteoking is a natural compound in traditional Chinese Medicine used to treat bone diseases; however, the effect of Osteoking on the differentiation of MSCs has not been reported. In this study, we aimed to investigate the effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells (rbMSCs).MethodsThe effects of Osteoking on the proliferation and differentiation of rbMSCs were investigated. Different concentrations of Osteoking were prepared, and its cytotoxicity was evaluated by CCK-8 assay. The expression of osteogenic and adipogenic genes were determined, and several staining methods were used to reveal the osteogenic and adipogenic differentiation potential of rbMSCs.ResultsOur results show that appropriate concentrations of Osteoking can enhance osteogenic differentiation of rbMSCs and reduce adipogenic differentiation without any effect on proliferation. This may be related to the changes in related gene expression.ConclusionOsteoking enhances osteogenic differentiation and inhibits adipogenic differentiation of rbMSCs. Therefore, Osteoking may have a therapeutic potential for treating bone disease caused by changes in differentiation function of MSCs.

引用

页数：9

共 21 条

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