Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins-rabbit IgG antibody system by whole-column imaged capillary isoelectric focusing

被引：18

作者：

Wu, XZ

Huang, TM

Mullett, WM

Yeung, JM

Pawliszyn, J

机构：

[1] Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada

[2] Natl Food Processors Assoc, Washington, DC 20005 USA

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 2001年 / 13卷 / 08期

关键词：

whole column imaging; capillary isoelectric focusing; peanut allergenic protein; rabbit IgG antibody; immunoreaction;

D O I：

10.1002/mcs.10013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The recently developed whole-column imaged capillary isoelectric focusing focusing is an advanced technique for the pI determination of proteins. In this study, we initially applied this technique to determine the pI of peanut allergens and its antibody, rabbit IgG. Two main proteins, Ara h I and Ara h II, were found in the peanut allergen solution. Their pI values were determined to be about 4.5-4.7 and 5.1-5.6, respectively. The value of pI for the antibody rabbit IgG was 6.1-6.5. Second, the immunoreaction selectivity of the two main peanut allergenic proteins with antibody rabbit IgG was studied. The CIEF results show that the peanut allergen Ara h II has a larger affinity for binding to the antibody rabbit IgG than Ara h I. (C) 2001 John Wiley & Sons, Inc.

引用

页码：322 / 326

页数：5

共 21 条

[1] IDENTIFICATION AND CHARACTERIZATION OF A 2ND MAJOR PEANUT ALLERGEN, ARA H II, WITH USE OF THE SERA OF PATIENTS WITH ATOPIC-DERMATITIS AND POSITIVE PEANUT CHALLENGE [J].