Shared antigenic epitopes and pathobiological functions of anti-p185her2/neu monoclonal antibodies

被引：40

作者：

Zhang, HT

Wang, Q

Montone, KT

Peavey, JE

Drebin, JA

Greene, MI

Murali, R

机构：

[1] Univ Penn, Sch Med, Dept Pathol, Philadelphia, PA 19104 USA

[2] Abington Mem Hosp, Dept Pathol, Abington, PA 19001 USA

[3] Washington Univ, Dept Surg, St Louis, MO 63110 USA

来源：

EXPERIMENTAL AND MOLECULAR PATHOLOGY | 1999年 / 67卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1006/exmp.1999.2266

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

We have studied two anti-p185 antibodies: the monoclonal antibody 7.16.4 and rhuMAb 4D5, which were raised against the the ectodomain of rat (p185(neu)), and the human (p185(her2/neu)) homolog, respectively. Studies on the structure of these two antibodies indicate that they share structural similarity in the variable region, especially the CDR3 region, which determines the antibody-antigen interaction. Further studies by flow cytometry revealed that 7.16.4 can compete with rhuMAb4DS for binding to the cell surface p185(her2/neu), suggesting that these two antibodies share an epitope on the p185 receptor. Furthermore, 7.16.4 can also inhibit proliferation and transformation caused by p185(her2/neu). Moreover the rhuMAb 4D5 binds to the rat p185(neu). With the observation that 7.16.4 positively stains human breast cancer tissues that overexpress p185(her2/neu), 7.16.4 may be useful for the pathological diagnosis and therapy of human tumors. (C) 1999 Academic Press.

引用

页码：15 / 25

页数：11

共 23 条

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