Identification of the Vibrio vulnificus wbpP gene and evaluation of its role in virulence

被引：28

作者：

Park, NY

Lee, JH

Kim, MW

Jeong, HG

Lee, BC

Kim, TS

Choi, SH ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Agr Biotechnol, Dept Food Sci & Technol, Ctr Agr Biomat, Seoul 151742, South Korea

[2] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

来源：

INFECTION AND IMMUNITY | 2006年 / 74卷 / 01期

关键词：

D O I：

10.1128/IAI.74.1.721-728.2006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A wbpP gene encoding a putative UDP-N-acetyl-D-glucosamine C-4 epimerase was identified and cloned from Vibrio vulnificus. The functions of the wbpP gene, assessed by the construction of an isogenic mutant and by evaluating its phenotype changes, demonstrated that WbpP is essential in both the pathogenesis and the capsular polysaccharide biosynthesis of V vulnificus.

引用

收藏

页码：721 / 728

页数：8

相关论文

共 37 条

[1] Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide [J].

Bélanger, M ;

Burrows, LL ;

Lam, JS .

MICROBIOLOGY-UK, 1999, 145 :3505-3521

[2] PRESENCE OF A CAPSULE IN VIBRIO-VULNIFICUS BIOTYPE-2 AND ITS RELATIONSHIP TO VIRULENCE FOR EELS [J].

BIOSCA, EG ;

LLORENS, H ;

GARAY, E ;

AMARO, C .

INFECTION AND IMMUNITY, 1993, 61 (05) :1611-1618

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] Classification of Vibrio vulnificus strains by the carbohydrate composition of their capsular polysaccharides [J].

Bush, CA ;

Patel, P ;

Gunawardena, S ;

Powell, J ;

Joseph, A ;

Johnson, JA ;

Morris, JG .

ANALYTICAL BIOCHEMISTRY, 1997, 250 (02) :186-195

[5] Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6 [J].

Creuzenet, C ;

Belanger, M ;

Wakarchuk, WW ;

Lam, JS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (25) :19060-19067

[6] MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS, PROMOTER PROBING, AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA [J].

DELORENZO, V ;

HERRERO, M ;

JAKUBZIK, U ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6568-6572

[7] Relation of capsular polysaccharide production and colonial cell organization to colony morphology in Vibrio parahaemolyticus [J].

Enos-Berlage, JL ;

McCarter, LL .

JOURNAL OF BACTERIOLOGY, 2000, 182 (19) :5513-5520

[8]

Gulig PA, 2005, J MICROBIOL, V43, P118

[9] CAPSULAR TYPES OF VIBRIO-VULNIFICUS - AN ANALYSIS OF STRAINS FROM CLINICAL AND ENVIRONMENTAL SOURCES [J].

HAYAT, U ;

REDDY, GP ;

BUSH, CA ;

JOHNSON, JA ;

WRIGHT, AC ;

MORRIS, JG .

JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (03) :758-762

[10] Differential expression of Vibrio vulnificus elastase gene in a growth phase-dependent manner by two different types of promoters [J].

Jeong, HS ;

Jeong, KC ;

Choi, HK ;

Park, KJ ;

Lee, KH ;

Rhee, JH ;

Choi, SH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (17) :13875-13880

← 1 2 3 4 →