Antioxidant properties of Erigeron annuus extract and its three phenolic constituents

被引:27
作者
Lee, HJ
Seo, Y [1 ]
机构
[1] Korea Maritime Univ, Div Marine Environm & Biosci, Pusan 606791, South Korea
[2] Korea Maritime Univ, RIMST, Pusan 606791, South Korea
基金
新加坡国家研究基金会;
关键词
Erigeron annuus; authentic peroxynitrite (ONOO-); 3-morpholinosydnonimine (SIN-1); 1,1-diphenyl-2-picrylhydrazyl radical (DPPH);
D O I
10.1007/BF02931862
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The antioxidant activity of the extract of Erigeron annuus was assessed by means of two different in vitro tests: bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test) and the scavenging of authentic peroxynitrite in company with peroxynitrite generation from 3-morpholinosydnonimine (SIN-1). In both tests, the 85% aq. MeOH and n-BuOH soluble fractions of the crude extract showed a significant scavenging effect on peroxynitrite and DPPH radical in comparison to L-ascorbic acid. And bioassay-guided fractionation of the n-BuOH soluble fraction led to the isolation of three compounds: Apigenin (1), quercetin-3-0-glucoside (2), and caffeic acid (3). The structures of the isolated compounds were elucidated on the basis of their spectroscopic data and their antioxiclant activities were measured by determining their capacity to scavenge peroxynitrite and the DPPH radical.
引用
收藏
页码:13 / 18
页数:6
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