RNA Exosome Depletion Reveals Transcription Upstream of Active Human Promoters

被引：555

作者：

Preker, Pascal ^{[1
]}

Nielsen, Jesper ^{[2
]}

Kammler, Susanne ^{[1
]}

Lykke-Andersen, Soren ^{[1
]}

Christensen, Marianne S. ^{[1
]}

Mapendano, Christophe K. ^{[1
]}

Schierup, Mikkel H. ^{[2
]}

Jensen, Torben Heick ^{[1
]}

机构：

[1] Univ Aarhus, Ctr mRNP Biogenesis & Metab, Dept Mol Biol, DK-8000 Aarhus C, Denmark

[2] Univ Aarhus, Bioinformat Res Ctr, DK-8000 Aarhus C, Denmark

来源：

SCIENCE | 2008年 / 322卷 / 5909期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1126/science.1164096

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Studies have shown that the bulk of eukaryotic genomes is transcribed. Transcriptome maps are frequently updated, but low- abundant transcripts have probably gone unnoticed. To eliminate RNA degradation, we depleted the exonucleolytic RNA exosome from human cells and then subjected the RNA to tiling microarray analysis. This revealed a class of short, polyadenylated and highly unstable RNAs. These promoter upstream transcripts ( PROMPTs) are produced similar to 0.5 to 2.5 kilobases upstream of active transcription start sites. PROMPT transcription occurs in both sense and antisense directions with respect to the downstream gene. In addition, it requires the presence of the gene promoter and is positively correlated with gene activity. We propose that PROMPT transcription is a common characteristic of RNA polymerase II ( RNAPII) transcribed genes with a possible regulatory potential.

引用

页码：1851 / 1854

页数：4

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