The intermediate layer: A morphologic study of the elastin and hyaluronic acid constituents of normal human vocal folds

被引:95
作者
Hammond, TH [1 ]
Zhou, RX [1 ]
Hammond, EH [1 ]
Pawlak, A [1 ]
Gray, SD [1 ]
机构
[1] UNIV UTAH,SCH MED,DEPT PATHOL,SALT LAKE CITY,UT
关键词
vocal fold; histology; image analysis; elastin; oxytalan; elaunin; hyaluronic acid; extracellular matrix; lamina propria; ultrastructure;
D O I
10.1016/S0892-1997(97)80024-0
中图分类号
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号
100104 ; 100213 ;
摘要
The lamina propria of vocal folds are important in voice production. We evaluated the morphologic features of elastin and hyaluronic acid, two important constituents of the lamina propria. Thirty normal human vocal folds were obtained from patients dying of traumatic causes without vocal fold injury. These tissues were immediately prepared for histologic and ultrastructural examination by standard methods. For specific study of the ultrastructure of the layers of the lamina propria, six vocal folds were divided horizontally through the midplane of the lamina propria. We found that the elastin composition of the vocal folds is variable, the largest amount being seen in the midportion on elastin-van Gieson (EVG) staining and ultrastructural evaluation. The superficial layer of the lamina propria contains fewer large elastin fibers. In this region, we found that elastin was predominantly composed of elaunin and oxytalan, which stain poorly with EVG. Using computer-assisted image analysis, we quantified the differences in elastin composition between the layers. The amount of elastin varied between men and women, and these differences could not be accurately measured by the methods employed. Hyaluronic acid was abundant especially in the midportion of the lamina propria and was significantly more abundant in men than women on quantification. The significance of these observations in normal vocal folds is discussed.
引用
收藏
页码:59 / 66
页数:8
相关论文
共 19 条
[11]   AGE-DEPENDENT CHANGES OF HYALURONAN IN HUMAN SKIN [J].
MEYER, LJM ;
STERN, R .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 102 (03) :385-389
[12]   THE EXTRACELLULAR PROCESSING AND CATABOLISM OF HYALURONAN IN CULTURED ADULT ARTICULAR-CARTILAGE EXPLANTS [J].
NG, CK ;
HANDLEY, CJ ;
PRESTON, BN ;
ROBINSON, HC .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 298 (01) :70-79
[13]  
PAWLAK A, 1995, ANN OTO RHINOL LARYN, V105, P6
[14]   ELASTIN IN HUMAN, BABOON, AND MOUSE-LIVER - AN IMMUNOHISTOCHEMICAL AND IMMUNOELECTRON MICROSCOPIC STUDY [J].
PORTO, LC ;
CHEVALLIER, M ;
PEYROL, S ;
GUERRET, S ;
GRIMAUD, JA .
ANATOMICAL RECORD, 1990, 228 (04) :392-404
[15]   EXTRACELLULAR-MATRIX ANALOGS AS CARRIERS FOR GROWTH-FACTORS - INVITRO FIBROBLAST BEHAVIOR [J].
ROY, F ;
DEBLOIS, C ;
DOILLON, CJ .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1993, 27 (03) :389-397
[16]   ASSOCIATION OF ELASTIN WITH OXYTALAN FIBERS OF THE DERMIS AND WITH EXTRACELLULAR MICROFIBRILS OF CULTURED SKIN FIBROBLASTS [J].
SCHWARTZ, E ;
FLEISCHMAJER, R .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1986, 34 (08) :1063-1068
[17]   INFRAGLOTTIC ASPECT OF CANINE VOCAL FOLD VIBRATION - EFFECT OF INCREASE OF MEAN AIR-FLOW RATE AND LENGTHENING OF VOCAL FOLD [J].
YUMOTO, E ;
KADOTA, Y ;
KUROKAWA, H .
JOURNAL OF VOICE, 1993, 7 (04) :311-318
[18]  
ZHOU RX, 1992, ANAL QUANT CYTOL, V14, P73
[19]  
P C ASS VOC PATH