Characterization of the human SDHD gene encoding the small subunit of cytochrome b (cybS) in mitochondrial succinate-ubiquinone oxidoreductase

被引：50

作者：

Hirawake, H

Taniwaki, M

Tamura, A

Amino, H

Tomitsuka, E

Kita, K

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Biomed Chem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Kyoto Prefectural Univ, Dept Internal Med 3, Kyoto 606, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1999年 / 1412卷 / 03期

关键词：

mitochondria; succinate dehydrogenase; cytochrome b; gene structure; SDHD; nuclear respiratory factor (NRF); (human);

D O I：

10.1016/S0005-2728(99)00071-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have mapped large (cybL) and small (cybS) subunits of cytochrome b in the succinate-ubiquinone oxidoreductase (complex II) of human mitochondria to chromosome 1q21 and 11q23, respectively (H. Hirawake et al., Cytogenet. Cell Genet. 79 (1997) 132-138). In the present study, the human SDHD gene encoding cybS was cloned and characterized. The gene comprises four exons and three introns extending over 19 kb. Sequence analysis of the 5' promoter region showed several motifs for the binding of transcription factors including nuclear respiratory factors NRF-1 and NRF-2 at positions -137 and -104, respectively. In addition to this gene, six pseudogenes of cybS were isolated and mapped on the chromosome. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：295 / 300

页数：6

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