Rhodamine phalloidin F-actin - Critical concentration versus tensile strength

被引：8

作者：

Adami, R

Choquet, D

Grazi, E

机构：

[1] Univ Ferrara, Dipartmento Biochim & Biol Mol, I-44100 Ferrara, Italy

[2] Univ Bordeaux, La Histol Embryol, UMR 5541, Bordeaux, France

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 263卷 / 01期

关键词：

actin; filament; critical concentration; yield-strength;

D O I：

10.1046/j.1432-1327.1999.00501.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanic and elastic properties of rhodamine phalloidin F-actin were investigated as a function of the ionic strength and in the absence of Mg2+. By increasing ionic strength from 3 to 19 mM, critical concentration decreased from 146 to 36 mM and the yield strength increased from 5.6 pN to 28.6 pN. At the ionic strength of 12-13 mM the elastic modulus by stretching increased by 330-430 kP.nm(-1) up to the break point, when it was 38-44.2 MP. The work required to break the filament, 403-439 kJ.m(-1) provides an estimate of the free energy of annealing of rhodamine phalloidin F-actin, the annealing constant being 2.8 x 10(74) M-1.

引用

页码：270 / 275

页数：6

共 21 条

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