Production of (R)-chiral alcohols by a hydrogen-transfer bioreduction with NADH-dependent Leifsonia alcohol dehydrogenase (LSADH)

被引：98

作者：

Inoue, K ^{[1
]}

Makino, Y ^{[1
]}

Itoh, N ^{[1
]}

机构：

[1] Toyama Prefectural Univ, Biotechnol Res Ctr, Toyama 9390398, Japan

来源：

TETRAHEDRON-ASYMMETRY | 2005年 / 16卷 / 15期

关键词：

D O I：

10.1016/j.tetasy.2005.06.036

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Alcohol dehydrogenase (LSADH) isolated from Leifsonia sp. S749 was used to produce (R)-chiral alcohols. The enzyme with a broad substrate range reduced various prochiral ketones and keto esters to yield optically active secondary alcohols with a high enantiomeric excess. LSADH transferred the pro-S hydrogen of NADH to the carbonyl moiety of phenyl trifluoromethyl ketone 13 through its re face to give (S)-1-pheilyl-2,2,2-trifluoroethatiol 40. LSADH was able to efficiently reproduce NADH when 2-propanol was used as a hydrogen donor in the reaction mixture. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2539 / 2549

页数：11

共 38 条

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