Immunoprecipitation of DNA-protein complexes cross-linked by cis-diamminedichloroplatinum

被引：13

作者：

Chichiarelli, S

Coppari, S

Turano, C

Eufemi, M

Altieri, F

Ferraro, A ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Ist Pasteur Fdn Cenci Bolognetti, Dipartimento Sci Biochim Alessandro Rossi Fanelli, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, CNR, Ctr Biol Mol, I-00185 Rome, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 302卷 / 02期

关键词：

immunoprecipitation; nuclear proteins; cross-linking; cis-diamminedichloroplatinum II;

D O I：

10.1006/abio.2001.5543

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For the study of in vitro and in vivo DNA-protein interactions, cross-linking reactions driven by UV or formaldehyde have been frequently used, followed by standard protocols of immunoprecipitation and analysis of the DNA isolated from the complexes. Here we present a basically modified method to analyze the DNA-protein cross-linked complexes obtained by an alternative cross-linking reagent. The innovations presented here include cross-linking by cis-diamminedichloroplatinum 11, a fast method to isolate DNA-protein complexes using gel-filtration chromatography, and a modified procedure to obtain specific immunocomplexes that can be analyzed either for DNA or for protein content. The application of this method to two nuclear proteins from chicken liver nuclei is described. (C) 2002 Elsevier Science (USA).

引用

页码：224 / 229

页数：6

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