Murine endothelial cells support fetal liver erythropoiesis and myelopoiesis via distinct interactions

Endothelial cells are an important component of the haemopoietic microenvironment. To investigate how endothelial cells are involved in haemopoiesis, two established murine endothelial cell lines were assayed in stromal cultures with fetal liver haemopoietic cells, Both endothelial cell lines allowed for the proliferation and differentiation of erythroid and monocyte-macrophage precursors, suggesting that support for haemopoiesis is a general property of endothelial cells. Erythropoiesis was dependent on the addition of erythropoietin (Epo), whereas myelopoiesis was independent of added Epo, Haemopoietic colonies developed in close contact with the endothelial cells. Erythroid colonies did not develop when transwell filters were used between the stroma and haemopoietic cells, or when conditioned medium was used in place of stromal cells, In contrast, monocyte-macrophage colonies formed in the presence of transwell filters or conditioned medium, Thus close cell contact is necessary for erythropoiesis but not myelopoiesis under these conditions. These results suggest that different molecular mechanisms are used by endothelial cells to support erythroid development and myeloid development in the mouse fetal liver.