Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice

被引：18

作者：

Nagatani, N

Takumi, S

Tomiyama, M

Shimada, T

Tamiya, E

机构：

[1] JAPAN ADV INST SCI & TECHNOL,SCH MAT SCI,TATSUNOKUCHI,ISHIKAWA 92312,JAPAN

[2] ISHIKAWA AGR COLL,RES INST AGR RESOURCES,LAB GENET RESOURCES,NONOICHI,ISHIKAWA 921,JAPAN

[3] NATL INST AGROBIOL RESOURCES,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT SCIENCE | 1997年 / 124卷 / 01期

关键词：

heat stress response; GFP reporter gene; rice embryo; image analyses; promoter of polyubiquitin gene; particle bombardment;

D O I：

10.1016/S0168-9452(96)04589-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have introduced the green fluorescent protein gene under the control of the maize ubiquitin promoter into rice embryos by microprojectile bombardment. Transient expression of GFP on the surface cells in the rice embryos depended on the localization of DNA coated gold particles. Bialaphos-resistant rice calli were produced by bombardment with a two plasmid mixture of pUbi-GFP and pDM302 carrying the coding region of the bar gene. Microscopic fluorescent image analyses indicated that the area of GFP expression increased and spreaded on the transgenic rice calli after heat stress. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：49 / 56

页数：8

共 27 条

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