Single nucleotide polymorphisms for integrative mapping in the Turkey (Meleagris gallopavo)

被引：11

作者：

Reed, KM ^{[1
]}

Hall, MK ^{[1
]}

Chaves, LD ^{[1
]}

Knutson, TP ^{[1
]}

机构：

[1] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA

来源：

ANIMAL BIOTECHNOLOGY | 2006年 / 17卷 / 01期

关键词：

Turkey; genetic linkage map; single nucleotide polymorphism;

D O I：

10.1080/10495390500461112

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

When multiple genetic maps exist for a species, integration of these maps requires a set of common markers be genotyped across the individual mapping populations. In the turkey, three genetic maps based on separate mapping populations are available. In this study, SNP-based markers were developed for integrating the cDNA/RFLP-based map (1) with microsatellite markers of the second-generation turkey genome map (2). Forty-eight primer sets were designed and tested and 33 (69%) correctly amplified turkey genomic DNA by PCR. Putative SNPs were detected in 20 (61%) of the amplified gene fragments, and 10 SNP markers were subsequently genotyped by PCR/RFLP for segregation analysis. Eight SNP markers were incorporated into the turkey genetic map.

引用

页码：73 / 80

页数：8

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