Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology

被引:213
作者
Guschin, DY
Mobarry, BK
Proudnikov, D
Stahl, DA
Rittmann, BE
Mirzabekov, AD
机构
[1] NORTHWESTERN UNIV,DEPT CIVIL ENGN,EVANSTON,IL 60208
[2] VA ENGELHARDT MOL BIOL INST,JOINT HUMAN GENOME PROGRAM,MOSCOW 117984,RUSSIA
[3] ARGONNE NATL LAB,JOINT HUMAN GENOME PROGRAM,ARGONNE,IL 60439
关键词
D O I
10.1128/AEM.63.6.2397-2402.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The utility of parallel hybridization of environmental nucleic acids to many oligonucleotides immobilized in a matrix of polyacrylamide gel pads on a glass slide (oligonucleotide microchip) was evaluated. Oligonucleotides complementary to small-subunit rRNA sequences of selected microbial groups, encompassing key genera of nitrifying bacteria, were shown to selectively retain labeled target nucleic acid derived from either DNA or RNA forms of the target sequences. The utility of varying the probe concentration to normalize hybridization signals and the use of multicolor detection for simultaneous quantitation of multiple probe-target populations were demonstrated.
引用
收藏
页码:2397 / 2402
页数:6
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