Molecular diversity and phylogeny of rhizobia associated with wild legumes native to Xinjiang, China

被引：38

作者：

Han, Tian Xu ^{[1
]}

Wang, En Tao ^{[1
,2
]}

Han, Li Li ^{[1
]}

Chen, Wen Feng ^{[1
]}

Sui, Xin Hua ^{[1
]}

Chen, Wen Xin ^{[1
]}

机构：

[1] China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol,Minist Agr, Key Lab Agromicrobial Resource & Applicat, Beijing 100193, Peoples R China

[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2008年 / 31卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Xinjiang; Wild legumes; Rhizobial diversity; Phylogeny; MLSA;

D O I：

10.1016/j.syapm.2008.04.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A total of 111 rhizobial strains were isolated from wild legumes in Xinjiang, an isolated region of northwest China. Nine genomic species belonging to four genera of Rhizobium, Mesorhizobium, Ensifer, and Bradyrhizobium were defined among these strains based on the characterization of amplified 16S ribosomal DNA restriction analysis (ARDRA), restriction fragment length polymorphism (RFLP) analysis of 16S-23S rDNA intergenic spacers (IGS), 16S rRNA gene sequencing and multilocus sequence analysis (MLSA). Twenty-five nodC types corresponding to eight phylogenetic clades were divided by RFLP and sequence analysis of the PCR-amplified nodC gene. The acid-producing Rhizobium and Mesorhizobium species were predominant, which may be related to both the local environments and the hosts sampled. The present study also showed the limitation of using nod genes to estimate the host specificity of rhizobia. (C) 2008 Elsevier GmbH. All rights reserved.

引用

页码：287 / 301

页数：15

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