THE NODC PROTEIN OF AZORHIZOBIUM-CAULINODANS IS AN N-ACETYLGLUCOSAMINYLTRANSFERASE

被引：146

作者：

GEREMIA, RA ^{[1
]}

MERGAERT, P ^{[1
]}

GEELEN, D ^{[1
]}

VANMONTAGU, M ^{[1
]}

HOLSTERS, M ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,KL LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 07期

关键词：

CHITIN SYNTHASE; NODULATION; RHIZOBIUM; SESBANIA-ROSTRATA;

D O I：

10.1073/pnas.91.7.2669

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nod factors are signal molecules produced by Azorhizobium, Bradyrhizobium, and Rhizobium species that trigger nodule formation in leguminous host plants. The backbone of Nod factors consists of a beta-1,4-N-acetylglucosamine oligosaccharide from which the N-acetyl group at the nonreducing end is replaced by a fatty acid. The nodABC gene products are necessary for backbone biosynthesis. By incubation of cell extracts from Azorhizobium caulinodans with radioactive uridine diphosphate-N-acetylglucosamine, Nod factor precursors were identified and characterized as beta-1,4-N-acetylglucosamine oligosaccharides. By analysis of different nod gene mutants and by expression of nodC in Escherichia coli, the N-acetylglucosaminyltransferase activity was ascribed to the NodC protein. The results suggest that the first step in biosynthesis of Nod factors is the assembly of the oligosaccharide chain.

引用

页码：2669 / 2673

页数：5

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