Automated SPE-RP-HPLC fractionation of biofluids combined to off-line NMR spectroscopy for biomarker identification in metabonomics

被引：24

作者：

Rezzi, Serge ^{[1
]}

Vera, Francia Arce ^{[1
]}

Martin, Francois-Pierre J. ^{[1
]}

Wang, Serena ^{[1
]}

Lawler, Dennis ^{[2
]}

Kochhar, Sunil ^{[1
]}

机构：

[1] Nestle Res Ctr, BioAnalyt Sci, CH-1000 Lausanne 26, Switzerland

[2] Nestle Res Ctr, Pet Basic Res, St Louis, MO 63164 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 871卷 / 02期

关键词：

solid phase extraction; high-pressure liquid chromatography; metabonomics; metabolomics; nuclear magnetic resonance spectroscopy; cat urine;

D O I：

10.1016/j.jchromb.2008.04.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR-based metabonomics is a valuable and straightforward approach to measuring hundreds of metabolites in complex biofluids. However, metabolite identification is sometimes limited by overlapped signals in NMR spectra. We describe a new methodology using an automated hyphenation of solid phase extraction (SPE) with RP-HPLC combined to NMR spectroscopy, which allowed identification of 72 metabolites of various molecular classes in human urine. This methodology was also Successfully applied to the fractionation of a cat urine sample to aid identification of aromatic Compounds and felinine. The SPE-RP-HPLC method appears to be a reliable tool to support biomarker discovery in metabonomic studies. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：271 / 278

页数：8

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