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Renal endothelin-converting enzyme in rats with congestive heart failure

被引：3
作者：
Abassi, Z
Winaver, T
Rubinstein, I
Shimada, K
Takahashi, M
Tanzawa, K
Hoffman, A
机构：
[1] Rambam Med Ctr, Dept Vasc Surg, IL-31096 Haifa, Israel
[2] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Physiol & Biophys, IL-31096 Haifa, Israel
[3] Sankyo Co Ltd, Biol Res Labs, Tokyo 140, Japan
来源：
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | 1998年 / 31卷
关键词：
endothelin-converting enzyme; congestive heart failure; renal cortex; endothelin;
D O I：
10.1097/00005344-199800001-00011
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The expression and immunoreactivity of endothelin-converting enzyme (ECE) were examined in the renal tissue of rats with experimental congestive heart failure (CHF). Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that ECE mRNA was more abundant (about twofold) in the renal medulla than in the cortex. Induction of heart failure caused a significant enhancement in the expression of this key enzyme in the renal cortex of rats with compensated CHF (Delta + 28%) and in animals with decompensated heart failure (Delta + 58%). An identical trend was also observed in the renal medulla, although these increases were moderate compared to those in the cortex. Similar findings were observed with Western blot techniques applying two monoclonal antibodies to rat ECE (AEC32-236 and AEC27-121). Taken together, these data suggest that upregulation of ECE is an important component in the activated renal ET system in CHF.
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页码：S31 / S34
页数：4
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