CDNA CLONING AND EXPRESSION OF BOVINE ENDOTHELIN-CONVERTING ENZYME

被引：75

作者：

IKURA, T

SAWAMURA, T

SHIRAKI, T

HOSOKAWA, H

KIDO, T

HOSHIKAWA, H

SHIMADA, K

TANZAWA, K

KOBAYASHI, S

MIWA, S

MASAKI, T

机构：

[1] KYOTO UNIV,GRAD SCH HUMAN & ENVIRONM STUDIES,DEPT NEUROSCI,KYOTO 606,JAPAN

[2] SANKYO CO LTD,BIOL RES LABS,TOKYO 140,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2343

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned cDNA encoding bovine endothelin converting enzyme (ECE). The predicted amino acid sequence of bovine ECE consisted of 758 amino acid residues. Northern blot analysis revealed that ECE mRNA was abundantly expressed in lung. Co-expression of the cloned cDNA of bovine ECE with human preproET-1 cDNA in CHO-K1 cells resulted in the production of mature ET-1 and this production was inhibited by phosphoramidon. (C) 1994 Academic Inc.

引用

页码：1417 / 1422

页数：6

共 14 条

[1]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[2] THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES [J].