Isoprenoid biosynthesis via the methylerythritol phosphate pathway:: accumulation of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate in a gcpE deficient mutant of Escheriehia coli

被引：22

作者：

Seemann, M

Campos, N

Rodríguez-Concepción, M

Hoeffler, JF

Grosdemange-Billiard, C

Boronat, A

Rohmer, M

机构：

[1] Univ Strasbourg, CNRS, Inst Le Bel, F-67070 Strasbourg, France

[2] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

来源：

TETRAHEDRON LETTERS | 2002年 / 43卷 / 05期

关键词：

biosynthesis; 2-C-methyl-D-erythritol; 4-phosphate; 2,4-cyclodiphosphate; gcpE; isoprenoids;

D O I：

10.1016/S0040-4039(01)02269-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

In the bacterium Escherichia coli. gcpE is an essential gene in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Incubation of [1-H-3]methylerythritol with an E. coli mutant defective in the gcpE gene resulted in the accumulation of [1-H-3]methylerythritol 2,4-cyclodiphosphate. This suggests that the GCPE protein is involved in the further conversion of methylerythritol cyclodiphosphate into isoprenoids. (C) 2002 Elsevier Science Ltd. All rights reserved.

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收藏

页码：775 / 778

页数：4

相关论文

共 29 条

[1] LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli [J].

Altincicek, B ;

Kollas, AK ;

Eberl, M ;

Wiesner, J ;

Sanderbrand, S ;

Hintz, M ;

Beck, E ;

Jomma, H .

FEBS LETTERS, 2001, 499 (1-2) :37-40

[2] GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli [J].

Altincicek, B ;

Kollas, AK ;

Sanderbrand, S ;

Wiesner, J ;

Hintz, M ;

Beck, E ;

Jomaa, H .

JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2411-2416

[3] Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate:: a novel system for the genetic analysis of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis [J].

Campos, N ;

Rodríguez-Concepción, M ;

Sauret-Güeto, S ;

Gallego, F ;

Lois, LM ;

Boronat, A .

BIOCHEMICAL JOURNAL, 2001, 353 :59-67

[4] Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli [J].

Campos, N ;

Rodríguez-Concepción, M ;

Seemann, M ;

Rohmer, M ;

Boronat, A .

FEBS LETTERS, 2001, 488 (03) :170-173

[5]

CHARON L, 2000, BIOCHEM J, V436, P737

[6] Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis [J].

Cunningham, FX ;

Lafond, TP ;

Gantt, E .

JOURNAL OF BACTERIOLOGY, 2000, 182 (20) :5841-5848

[7] The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms [J].

Eisenreich, W ;

Schwarz, M ;

Cartayrade, A ;

Arigoni, D ;

Zenk, MH ;

Bacher, A .

CHEMISTRY & BIOLOGY, 1998, 5 (09) :R221-R233

[8] PROKARYOTIC HOPANOIDS - THE BIOSYNTHESIS OF THE BACTERIOHOPANE SKELETON - FORMATION OF ISOPRENIC UNITS FROM 2 DISTINCT ACETATE POOLS AND A NOVEL TYPE OF CARBON CARBON LINKAGE BETWEEN A TRITERPENE AND D-RIBOSE [J].

FLESCH, G ;

ROHMER, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 175 (02) :405-411

[9] Biosynthesis of terpenoids:: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate [J].

Herz, S ;

Wungsintaweekul, J ;

Schuhr, CA ;

Hecht, S ;

Lüttgen, H ;

Sagner, S ;

Fellermeier, M ;

Eisenreich, W ;

Zenk, MH ;

Bacher, A ;

Rohdich, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2486-2490

[10] Synthesis of tritium labelled 2-C-methyl-D-erythritol, a useful substrate for the elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis [J].

Hoeffler, JF ;

Grosdemange-Billiard, C ;

Rohmer, M .

TETRAHEDRON LETTERS, 2000, 41 (25) :4885-4889