Rapid identification and cloning of bacterial transferrin and lactoferrin receptor protein genes

被引:18
作者
Ogunnariwo, JA
Schryvers, AB
机构
[1] Dept. of Microbiol. and Infect. Dis., University of Calgary, Calgary, Alta.
关键词
D O I
10.1128/jb.178.24.7326-7328.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The sequences of genes encoding the transferrin and lactoferrin receptor proteins from several bacterial species were analyzed for areas of identity ire the predicted protein sequences. Degenerate oligonucleotide primers were designed and tested for their ability to amplify portions of the receptor genes, Primer pairs capable of amplifying products of the tbpA/lbpA or tbpB/lbpB genes from all specie possessing these receptors were identified.
引用
收藏
页码:7326 / 7328
页数:3
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