A gene expression screen in zebrafish embryogenesis

被引：172

作者：

Kudoh, T

Tsang, M

Hukriede, NA

Chen, XF

Dedekian, M

Clarke, CJ

Kiang, A

Schultz, S

Epstein, JA

Toyama, R

Dawid, IB ^{[1
]}

机构：

[1] NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA

[2] NICHHD, Unit Biol Computat, NIH, Bethesda, MD 20892 USA

来源：

GENOME RESEARCH | 2001年 / 11卷 / 12期

关键词：

D O I：

10.1101/gr.209601

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A screen for developmentally regulated genes was conducted in the zebrafish, a system offering substantial advantages for the study of the molecular genetics of vertebrate embryogenesis. Clones from a normalized cDNA library from early somitogenesis stages were picked randomly and tested by high-throughput in situ hybridization for restricted expression in at least one of four stages of development. Among 2765 clones that were screened, a total of 347 genes with patterns judged to be restricted were selected. These clones were subjected to partial sequence analysis, allowing recognition of functional motifs in 163 among them. In addition, a portion of the clones were mapped with the aid of the LN54 radiation hybrid panel. The usefulness of the in situ hybridization screening approach is illustrated by describing several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different regions of the developing brain.

引用

页码：1979 / 1987

页数：9

共 48 条

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