Isolation and functional characterization of a transcription factor VpNAC1 from Chinese wild Vitis pseudoreticulata

被引:64
作者
Zhu, Ziguo [1 ,2 ,3 ]
Shi, Jiangli [1 ,2 ,3 ]
He, Mingyang [1 ,2 ,3 ]
Cao, Jiangling [1 ,2 ,3 ]
Wang, Yuejin [1 ,2 ,3 ]
机构
[1] NW A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
[2] Minist Agr, Key Lab Biol & Genet Improvement Hort Crops NW Re, Yangling, Peoples R China
[3] NW A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Biotic stress; Resistance; Grapevine; NAC transcription factor; Vitis pseudoreticulata; GENE-EXPRESSION; DEFENSE RESPONSES; JASMONIC ACID; ARABIDOPSIS; RESISTANCE; REGULATOR;
D O I
10.1007/s10529-012-0890-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 090105 [作物生产系统与生态工程];
摘要
NAC (for NAM, ATAF1, 2, and CUC2) family genes encode plant-specific transcription factors that play important roles in plant development regulation and in abiotic and biotic stresses. However, the function of NAC genes in grapevines is not clear. A novel NAC transcription factor, designated as VpNAC1, was isolated from Chinese wild Vitis pseudoreticulata. It belongs to the TERN subgroup and is a nuclear-targeting protein and functions as a transcriptional activator. Moreover, VpNAC1 was induced by the fungus Erysiphe necator and the exogenous hormones, particularly salicylic acid, methyl jasmonate and ethylene. Over-expression of VpNAC1 in tobacco plants enhanced their resistance to Erysiphe cichoracearum and Phytophthora parasitica var. nicotianae Tucker. These results suggest that VpNAC1 acts as a positive regulator in biotic stresses.
引用
收藏
页码:1335 / 1342
页数:8
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