Characterization of a large glycoprotein proteoglycan by size-exclusion chromatography combined with light and X-ray scattering methods

被引：8

作者：

Watanabe, Yasushi ^{[1
]}

Inoko, Yoji ^{[2
]}

机构：

[1] NARO, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[2] Osaka Univ, Grad Sch Engn Sci, Toyonaka, Osaka 5608531, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1303卷

基金：

日本学术振兴会;

关键词：

Light scattering; Small-angle X-ray scattering; SEC-SAXS; Molecular weight; Conformation; Proteoglycan; PERFORMANCE GEL CHROMATOGRAPHY; MEMBRANE-PROTEIN PORIN; ANGLE; CARTILAGE; WEIGHT;

D O I：

10.1016/j.chroma.2013.06.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular weight and chain conformation of a proteoglycan derived from shark cartilage in solution were characterized by size-exclusion chromatography combined with low-angle laser light scattering and small-angle X-ray scattering methods. The total molecular weight of the proteoglycan was 3.9 +/- 0.2 million and the molecular weight of the main component was about 2.0 +/- 0.2 million. The X-ray scattering data revealed that the main components of the proteoglycan are nearly equal to a chain with excluded volume and their persistence lengths range from 13.5 to 16.4 nm. These results show that size-exclusion chromatography combined with low-angle laser light scattering and small-angle X-ray scattering measurements are complementarily useful for characterization of large biopolymers in solution. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：100 / 104

页数：5

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