Reverse line blot assay for direct identification of seven streptococcus agalactiae major surface protein antigen genes

被引:18
作者
Zhao, ZT
Kong, FR
Gilbert, GL
机构
[1] Inst Clin Pathol & Med Res, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
[2] Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
关键词
D O I
10.1128/CVI.13.1.145-149.2006
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We developed a multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB) to detect the genes encoding members of the family of variable surface-localized proteins of Streptococcus agalactiae (group B streptococcus [GBS]), namely, Bca (C alpha), Rib, Epsilon (Epsilon/Alp1/Alp5), Alp2, Alp3, and Alp4, and the immunoglobulin A binding protein, Bac (C beta). We used the assay to identify these genes in a collection of well-characterized GBS isolates and reference strains. The results showed that mPCR/RLB avoids the common problems of cross-reaction and nontypability associated with protein typing using antisera. It is as sensitive as, but more practical than, separate gene-specific PCRs and would be suitable for large molecular epidemiological studies of GBS.
引用
收藏
页码:145 / 149
页数:5
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