Amino-containing cationic latex-oligodeoxyribonucleotide conjugates:: application to diagnostic test sensitivity enhancement

被引：67

作者：

Delair, T ^{[1
]}

Meunier, F ^{[1
]}

Elaïssari, A ^{[1
]}

Charles, MH ^{[1
]}

Pichot, C ^{[1
]}

机构：

[1] Ecole Normale Super Lyon, Unite Mixte CNRS BioMerieux, F-69364 Lyon 07, France

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 1999年 / 153卷 / 1-3期

关键词：

aminated polystyrene; poly(N-isopropylacrylamide); oligodeoxyribonucleotide; adsorption; grafting; diagnostic;

D O I：

10.1016/S0927-7757(98)00456-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The covalent immobilization of nucleic acid probes (ODN) onto amino-containing cationic hydrophobic (polystyrene) or hydrophilic poly(N-isopropylacrylamide) latexes has been investigated. A preliminary study was devoted to the adsorption behavior of ODNs as a function of pH, ionic strength and type of latex. A drastic reduction of ODN adsorption upon increasing ionic strength was found, especially with hydrophilic-based latexes. Then, covalent attachment of ODNs onto the reactive particles was performed in a basic pH(> 9) so as to avoid physical adsorption. Coating of the ODN-latex conjugates onto solid surfaces resulted in the formation of two dimensional assemblies. These functionalized surfaces were finally used for the detection of the HBV virus DNA, providing sensitivity improvement identical to what was already observed with linear polymers. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：341 / 353

页数：13

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