Application of the multi-colour FISH to interphase nuclei and metaphase spreads for simultaneous examination of monosomy 7 and trisomies 8 and 11 in acute myelocytic leukaemia (AML)

被引：4

作者：

Acar, H ^{[1
]}

Acar, A ^{[1
]}

机构：

[1] UNIV SELCUK,DEPT MED GENET,KONYA,TURKEY

来源：

CLINICAL AND LABORATORY HAEMATOLOGY | 1997年 / 19卷 / 01期

关键词：

acute myeloid leukaemia; fluorescence in situ hybridization; monosomy; 7; trisomy 8 and trisomy 11;

D O I：

10.1046/j.1365-2257.1997.00186.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have used the multi-colour (three) fluorescence in situ hybridization (FISH) technique based on the ratio labelling for the detection of monosomy 7 and trisomies 8 and 11 in 13 cases of acute myeloid leukaemia (AML). Two out of the 13 AML cases showed monosomy 7 and two out of the remaining cases exhibited trisomy 8 in interphase nuclei. Three of these results were confirmed by metaphase-FISH study. Trisomy 11 was not found either by the interphase FISH study or in the metaphase FISH study. These results demonstrate the potential power of multi-colour FISH using ratio labelling to produce more fluorescein colour in interphase nuclei for the detection of aneuploidies in leukaemia.

引用

页码：33 / 38

页数：6

共 25 条

[1]

ANASTASI J, 1993, BLOOD, V81, P1580

[2] DETECTION OF THE PHILADELPHIA-CHROMOSOME IN INTERPHASE NUCLEI [J].

ARNOLDUS, EPJ ;

WIEGANT, J ;

NOORDERMEER, IA ;

WESSELS, JW ;

BEVERSTOCK, GC ;

GROSVELD, GC ;

VANDERPLOEG, M ;

RAAP, AK .

CYTOGENETICS AND CELL GENETICS, 1990, 54 (3-4) :108-&

[3]

BARANGER L, 1990, LEUKEMIA, V4, P345

[4]

BAURMANN H, 1993, LEUKEMIA, V7, P334

[5]

BRIZARD F, 1994, LEUKEMIA, V8, P1005

[6] MULTIPLE COLORS BY FLUORESCENCE INSITU HYBRIDIZATION USING RATIO-LABELED DNA PROBES CREATE A MOLECULAR KARYOTYPE [J].

DAUWERSE, JG ;

WIEGANT, J ;

RAAP, AK ;

BREUNING, MH ;

VANOMMEN, GJB .

HUMAN MOLECULAR GENETICS, 1992, 1 (08) :593-598

[7] DOUBLE INSITU HYBRIDIZATION IN COMBINATION WITH DIGITAL IMAGE-ANALYSIS - A NEW APPROACH TO STUDY INTERPHASE CHROMOSOME TOPOGRAPHY [J].

EMMERICH, P ;

LOOS, P ;

JAUCH, A ;

HOPMAN, AHN ;

WIEGANT, J ;

HIGGINS, MJ ;

WHITE, BN ;

VANDERPLOEG, M ;

CREMER, C ;

CREMER, T .

EXPERIMENTAL CELL RESEARCH, 1989, 181 (01) :126-140

[8]

HEEREMA NA, 1993, LEUKEMIA, V7, P537

[9]

Heim S, 1987, CANCER CYTOGENETICS, P65

[10]

JENKINS RB, 1992, BLOOD, V79, P3307

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