CRISPR/Cas9 and Genome Editing in Drosophila

被引：139

作者：

Bassett, Andrew R. ^{[1
]}

Liu, Ji-Long ^{[1
]}

机构：

[1] Univ Oxford, Dept Physiol Anat & Genet, MRC Funct Genom Unit, Oxford OX1 3QX, England

来源：

JOURNAL OF GENETICS AND GENOMICS | 2014年 / 41卷 / 01期

基金：

英国医学研究理事会; 欧洲研究理事会;

关键词：

Drosophila melanogaster; CRISPR; Cas9; Genome engineering; Targeted mutagenesis; ZINC-FINGER NUCLEASES; SHORT PALINDROMIC REPEATS; GENE DISRUPTION PROJECT; HOMOLOGOUS RECOMBINATION; TRANSCRIPTION FACTORS; HUMAN-CELLS; SACCHAROMYCES-CEREVISIAE; CHROMOSOMAL DELETIONS; DNA RECOGNITION; BINDING SITES;

D O I：

10.1016/j.jgg.2013.12.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in our ability to design DNA binding factors with specificity for desired sequences have resulted in a revolution in genetic engineering, enabling directed changes to the genome to be made relatively easily. Traditional techniques for generating genetic mutations in most organisms have relied on selection from large pools of randomly induced mutations for those of particular interest, or time-consuming gene targeting by homologous recombination. Drosophila melanogaster has always been at the forefront of genetic analysis, and application of these new genome editing techniques to this organism will revolutionise our approach to performing analysis of gene function in the future. We discuss the recent techniques that apply the CRISPR/Cas9 system to Drosophila, highlight potential uses for this technology and speculate upon the future of genome engineering in this model organism.

引用

页码：7 / 19

页数：13

共 88 条

[21] TOWARD A CODE FOR THE INTERACTIONS OF ZINC FINGERS WITH DNA - SELECTION OF RANDOMIZED FINGERS DISPLAYED ON PHAGE [J].

CHOO, Y ;

KLUG, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :11163-11167

[22]

CHOULIKA A, 1995, MOL CELL BIOL, V15, P1968

[23] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

[24] Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome [J].

Dahlem, Timothy J. ;

Hoshijima, Kazuyuki ;

Jurynec, Michael J. ;

Gunther, Derrick ;

Starker, Colby G. ;

Locke, Alexandra S. ;

Weis, Allison M. ;

Voytas, Daniel F. ;

Grunwald, David Jonah .

PLOS GENETICS, 2012, 8 (08)

[25] Promiscuous RNA binding by Polycomb repressive complex 2 [J].

Davidovich, Chen ;

Zheng, Leon ;

Goodrich, Karen J. ;

Cech, Thomas R. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2013, 20 (11) :1250-U273

[26] THE FUNCTION OF INDIVIDUAL ZINC FINGERS IN SEQUENCE-SPECIFIC DNA RECOGNITION BY TRANSCRIPTION FACTOR-IIIA [J].

DELRIO, S ;

MENEZES, SR ;

SETZER, DR .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 233 (04) :567-579

[27] Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems [J].

DiCarlo, James E. ;

Norville, Julie E. ;

Mali, Prashant ;

Rios, Xavier ;

Aach, John ;

Church, George M. .

NUCLEIC ACIDS RESEARCH, 2013, 41 (07) :4336-4343

[28] Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination [J].

Dickinson, Daniel J. ;

Ward, Jordan D. ;

Reiner, David J. ;

Goldstein, Bob .

NATURE METHODS, 2013, 10 (10) :1028-+

[29] An integrated encyclopedia of DNA elements in the human genome [J].

Dunham, Ian ;

Kundaje, Anshul ;

Aldred, Shelley F. ;

Collins, Patrick J. ;

Davis, CarrieA. ;

Doyle, Francis ;

Epstein, Charles B. ;

Frietze, Seth ;

Harrow, Jennifer ;

Kaul, Rajinder ;

Khatun, Jainab ;

Lajoie, Bryan R. ;

Landt, Stephen G. ;

Lee, Bum-Kyu ;

Pauli, Florencia ;

Rosenbloom, Kate R. ;

Sabo, Peter ;

Safi, Alexias ;

Sanyal, Amartya ;

Shoresh, Noam ;

Simon, Jeremy M. ;

Song, Lingyun ;

Trinklein, Nathan D. ;

Altshuler, Robert C. ;

Birney, Ewan ;

Brown, James B. ;

Cheng, Chao ;

Djebali, Sarah ;

Dong, Xianjun ;

Dunham, Ian ;

Ernst, Jason ;

Furey, Terrence S. ;

Gerstein, Mark ;

Giardine, Belinda ;

Greven, Melissa ;

Hardison, Ross C. ;

Harris, Robert S. ;

Herrero, Javier ;

Hoffman, Michael M. ;

Iyer, Sowmya ;

Kellis, Manolis ;

Khatun, Jainab ;

Kheradpour, Pouya ;

Kundaje, Anshul ;

Lassmann, Timo ;

Li, Qunhua ;

Lin, Xinying ;

Marinov, Georgi K. ;

Merkel, Angelika ;

Mortazavi, Ali .

NATURE, 2012, 489 (7414) :57-74

[30]

Esvelt KM, 2013, NAT METHODS, V10, P1116, DOI [10.1038/NMETH.2681, 10.1038/nmeth.2681]

← 1 2 3 4 5 6 7 8 9 →