Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

被引：231

作者：

He, Nianwu ^{[1
]}

Yang, Xingbin ^{[1
]}

Jiao, Yadong ^{[1
]}

Tian, Lingmin ^{[2
]}

Zhao, Yan ^{[3
]}

机构：

[1] Shaanxi Normal Univ, Key Lab, Minist Educ Med Resource & Nat Pharmaceut Chem, Coll Food Engn & Nutr Sci, Xian 710062, Peoples R China

[2] Wageningen Univ, Food Chem Lab, NL-6703 HD Wageningen, Netherlands

[3] Fourth Mil Med Univ, Sch Pharm, Xian 710032, Peoples R China

来源：

FOOD CHEMISTRY | 2012年 / 133卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Wolfberry fruit; Polysaccharides; Monosaccharide composition; Antioxidant and antitumor effects; LYCIUM-BARBARUM POLYSACCHARIDES; BREAST-CANCER CELLS; APOPTOSIS; EXTRACTION; GROWTH; PREVENTION; CRANBERRY; ARREST;

D O I：

10.1016/j.foodchem.2012.02.018

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Wolfberry fruit polysaccharides (WFPs) were isolated by hot-water extraction and ethanol precipitation. With HPLC analysis, WFPs were for the first time identified as acidic polysaccharides with galacturonic acid being the main component monosaccharide (24.9%), followed by galactose (21.3%), arabinose (18.5%), and glucose (15.9%), accounting for up to 80.6% of the molar percentage. WFPs exhibited a high antioxidant activity and a dose-dependent antiproliferative effect, with IC50 values of 134.9, 70.1 and 138.4 mu g/mL against A549, MCF-7 and LoVo cancer cells after 48 h of incubation as estimated by MTT assay, respectively. Flow cytometry analysis showed that WFPs exerted a stimulatory effect on apoptosis of MCF-7 cells, and induced the cell-cycle arrest at the G0/G1 phase, with the observation of intracellular ROS production and DNA damage. The present study demonstrated that these polysaccharides might have the potential to provide significant natural defence against human cancer. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：978 / 989

页数：12

共 30 条

[1]

Antioxidant, Antiproliferative, and Pro-apoptotic Capacities of Pentacyclic Triterpenes Found in the Skin of Olives on MCF-7 Human Breast Cancer Cells and Their Effects on DNA Damage [J].