Animal cell-death suppressors Bcl-xL and Ced-9 inhibit cell death in tobacco plants

被引:140
作者
Mitsuhara, I
Malik, KA
Miura, M
Ohashi, Y
机构
[1] Natl Inst Agrobiol Resources, Dept Mol Genet, Tsukuba, Ibaraki 3058602, Japan
[2] Japan Sci & Technol Corp, CREST, Chiyoda Ku, Tokyo 1010062, Japan
[3] Osaka Univ, Sch Med, Ctr Biomed Res, Dept Neuroanat, Suita, Osaka 565087, Japan
关键词
D O I
10.1016/S0960-9822(99)80341-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In plants, events similar to programmed cell death have been reported [1,2], although little is known of their mechanisms at the molecular level. To investigate the mechanism(s) involved, we overexpressed bcl-x(L), which encodes a mammalian suppressor of programmed cell death, in tobacco plants, under the control of a strong promoter [3]. In plants expressing Bcl-x(L), cell death induced by UV-B irradiation, paraquat treatment or the hypersensitive reaction (HR) to tobacco mosaic virus (TMV) infection was suppressed. The extent of suppression of cell death depended on the amount of Bcl-x(L) protein expressed, Similar enhanced resistance to cell death was found in transgenic tobacco plants overexpressing the ced-9 gene, a Caenorhabditis elegans homolog of bcl-x(L) [4], indicating that Bcl-x(L) and Ced-9 can function to inhibit cell death in plants.
引用
收藏
页码:775 / 778
页数:4
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